[Histonet] (no subject)
nbhatia <@t> dermatology.wisc.edu
Wed Jan 10 15:58:31 CST 2007
Generator Microsoft Word 11 (filtered medium) Hello Everyone,
I have been trying to stain paraffin embedded skin tissue from transgenic mice for HA tag expression to no avail.
Has anyone been successful for this.
These mice also express b-gal. Can I stain these sections for b-gal expression using the antibody. If so what method of antigen retrieval should I use as heat will destroy this enzyme.
Neehar Bhatia, Ph.D
Department of Dermatology,
University of Wisconsin,
445 Henry Mall, Rm 302,
Madison WI 53706
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