[Histonet] (no subject)

[Neehar Bhatia]

Generator Microsoft Word 11 (filtered medium) Hello Everyone, 
I have been trying to stain paraffin embedded skin tissue from transgenic mice for HA tag expression to no avail.
Has anyone been successful for this.
These mice also express b-gal. Can I stain these sections for b-gal expression using the antibody. If so what method of antigen retrieval should I use as heat will destroy this enzyme.
Please help
Thanks
Neehar Bhatia



Neehar Bhatia, Ph.D
Assistant Scientist,
Department of Dermatology,
University of Wisconsin,
445 Henry Mall, Rm 302,
Madison WI 53706
Ph 608.262.3239
Fax 608.263.2919