[Histonet] sheep CC10
Melissa Mazan
melissa.mazan <@t> tufts.edu
Tue Jan 9 11:31:22 CST 2007
Does anyone know of an antibody to CC10 that can be used on sheep
tissue? Thanks - Melissa Mazan
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> Today's Topics:
>
> 1. Re: memorandum markers (Rene J Buesa)
> 2. Parathyroid Storage (Weems, Joyce)
> 3. RE: memorandum markers (Greer, Patricia (CDC/CCID/NCZVED))
> 4. RE: memorandum markers (Horn, Hazel V)
> 5. F4/80 in FFPE mouse tissues (Horn, Diane A)
> 6. Special stain on fixed cytology smear (eileen dusek)
> 7. RE: Special stain on fixed cytology smear (Tony Henwood)
> 8. RE: Special stain on fixed cytology smear (Weems, Joyce)
> 9. RE: Special stain on fixed cytology smear (Douglas D Deltour)
> 10. Sheep CD31 (AGrobe2555 <@t> aol.com)
> 11. RUO OR ASR (Akemi Allison-Tacha)
> 12. RE: RUO OR ASR (Weems, Joyce)
> 13. RE: RUO OR ASR (Morken, Tim)
> 14. RE: RUO OR ASR (Weems, Joyce)
> 15. Re: Sheep CD31 (Gayle Callis)
> 16. need protocol for Neu N IHC stain (Maria Mejia)
> 17. Processing with Clear-Rite 3 (LeVier, Rebecca J)
> 18. Microinjection whole-mount immunolabelling (gloria @home)
> 19. B-Plus Fixative (Jessica Piche)
> 20. B-Plus Fixative (Jessica Piche)
> 21. Re: B-Plus Fixative (Richard Cartun)
> 22. Re: B-Plus Fixative (Richard Cartun)
> 23. RE: CD 10 protocol (Malam Jacqueline)
> 24. RUO or ASR (Akemi Allison-Tacha)
> 25. Hooray for Histotechs! (have to brag just a little)
> (Andrea Grantham)
> 26. Re: Special stain on fixed cytology smear (Rene J Buesa)
> 27. Re: B-Plus Fixative (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 8 Jan 2007 10:22:09 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] memorandum markers
> To: srishan <@t> mail.holyname.org, Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20070108182209.2894.qmail <@t> web61218.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I used to prepare them myself using 3x5 reference cards, cut to measure.
> A "productive" way of using some spare time (or by son auxiliary personnel).
> René J.
>
> srishan <@t> mail.holyname.org wrote:
>
> Hi,
>
> Happy New Year to all.
>
> I am looking to order memorandum markers, the dividers used in the slide
> file cabinets. Any one knows where I could order it?
>
> Thanks in advance
>
> Nirmala Srishan
> Histology Supervisor
> Holy Name Hospital
> Teaneck, NJ 07666
>
>
> _______________________________________________
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> ------------------------------
>
> Message: 2
> Date: Mon, 8 Jan 2007 14:17:17 -0500
> From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Subject: [Histonet] Parathyroid Storage
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F0E <@t> sjhaexc02.sjha.org>
> Content-Type: text/plain; charset="utf-8"
>
> I am in search of a facility that will store parathyroids. Our
> neighbor hospital has known of two locations but they are closing and
> will not accept the specimen that is scheduled to be removed in
> tomorrow's surgery. Any help (STAT!) would be much appreciated.
>
> Thanks,
> Joyce
>
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 404-851-7376 - Phone
> 404-851-7831 - Fax
>
>
>
> Confidentiality Notice ** The information contained in this message
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> ------------------------------
>
> Message: 3
> Date: Mon, 8 Jan 2007 14:52:31 -0500
> From: "Greer, Patricia \(CDC/CCID/NCZVED\)" <pwg1 <@t> cdc.gov>
> Subject: RE: [Histonet] memorandum markers
> To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, <srishan <@t> mail.holyname.org>,
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CB857F6460D42E4AAEA195054A25406C0D5F50F1 <@t> m-ncid-2.ncid.cdc.gov>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We go to a local print shop and ask them to cut card stock at 3 1/4 X
> 1 inch (the slide drawers had aren't very deep - you may want 3 1/2
> inch length). We get about 4000 at a time and they are much cheaper
> that ordering them from some of the lab supplies.
>
> Pat Greer
> Infectious Disease Pathology Branch
> Centers for Disease Control and Prevention
> Mail Stop G-32
> Atlanta, GA 30333
>
> 404-639-2811
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene
> J Buesa
> Sent: Monday, January 08, 2007 1:22 PM
> To: srishan <@t> mail.holyname.org; Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] memorandum markers
>
> I used to prepare them myself using 3x5 reference cards, cut to measure.
> A "productive" way of using some spare time (or by son auxiliary personnel).
> René J.
>
> srishan <@t> mail.holyname.org wrote:
>
> Hi,
>
> Happy New Year to all.
>
> I am looking to order memorandum markers, the dividers used in the
> slide file cabinets. Any one knows where I could order it?
>
> Thanks in advance
>
> Nirmala Srishan
> Histology Supervisor
> Holy Name Hospital
> Teaneck, NJ 07666
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
>
>
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>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 8 Jan 2007 14:35:54 -0600
> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> Subject: RE: [Histonet] memorandum markers
> To: srishan <@t> mail.holyname.org, Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F56 <@t> EMAIL.archildrens.org>
> Content-Type: text/plain; charset=us-ascii
>
> You can order them from LabStorage Systems. 1.800.345.4167
>
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Histology
> Arkansas Children's Hospital
> 800 Marshall Slot 820
> Little Rock, AR 72202
>
> phone 501.364.4240
> fax 501.364.3912
>
> visit us on the web at: www.archildrens.org
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> srishan <@t> mail.holyname.org
> Sent: Monday, January 08, 2007 11:35 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] memorandum markers
>
>
> Hi,
>
> Happy New Year to all.
>
> I am looking to order memorandum markers, the dividers used in the slide
> file cabinets. Any one knows where I could order it?
>
> Thanks in advance
>
> Nirmala Srishan
> Histology Supervisor
> Holy Name Hospital
> Teaneck, NJ 07666
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------------------------------------------------------
> The information contained in this message may be privileged and
> confidential and protected from disclosure. If the reader of this
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> you are hereby notified that any dissemination, distribution or
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> replying to the message and deleting it from your computer. Thank you.
> ==============================================================================
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>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 8 Jan 2007 14:41:58 -0600
> From: "Horn, Diane A" <hornd <@t> uthscsa.edu>
> Subject: [Histonet] F4/80 in FFPE mouse tissues
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <819189266B78D543A02D9D3956EE4F6C0484AC2D <@t> addax.win.uthscsa.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> I am searching for some advice on IHC with F4/80 on mouse tissues. The
> antibody is from Serotec diluted 1:50 in 2% BSA/PBS. The kidney tissues
> have been fixed in 4% paraformaldehyde and the liver has been fixed in
> 10% NFB. The liver stains fine but the kidney has high background and
> no positive staining for F4/80. I am using a kit from Biocare
> (rat-on-mouse) that is a biotin free system. The only other thing is
> the kidney sections were cut about 1-2 months ago and stored in a slide
> box in the -20 freezer (not frost free). Any advice or protocol would
> be appreciated!
>
>
>
> Thanks to all,
>
> Diane
>
>
>
> Diane A. Horn
>
> Department of Pathology
>
> UTHSCSA
>
> 7703 Floyd Curl Dr.
>
> San Antonio, TX 78229
>
> 210-567-4039
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 8 Jan 2007 13:18:12 -0800 (PST)
> From: eileen dusek <eileen_dusek <@t> yahoo.com>
> Subject: [Histonet] Special stain on fixed cytology smear
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Cc: me <e_dusek1 <@t> comcast.net>
> Message-ID: <20070108211812.11717.qmail <@t> web50602.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi Everyone,
> Happiest of New Year
>
> A quick cytology question.
> Can Pneumocysytis and GMS be stained on an alcohol fixed smear?
>
> I appreciate your help
>
> Eileen Dusek
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
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> ------------------------------
>
> Message: 7
> Date: Tue, 9 Jan 2007 08:32:42 +1100
> From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] Special stain on fixed cytology smear
> To: "eileen dusek" <eileen_dusek <@t> yahoo.com>, "histonet"
> <histonet <@t> lists.utsouthwestern.edu>
> Cc: me <e_dusek1 <@t> comcast.net>
> Message-ID: <B9EAF61856077F47BF9BE2F89AFC55520127C873 <@t> hedwig.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Yep,
> Sure can
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: 612 9845 3306
> Fax: 612 9845 3318
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of eileen
> dusek
> Sent: Tuesday, 9 January 2007 8:18 AM
> To: histonet
> Cc: me
> Subject: [Histonet] Special stain on fixed cytology smear
>
>
> Hi Everyone,
> Happiest of New Year
>
> A quick cytology question.
> Can Pneumocysytis and GMS be stained on an alcohol fixed smear?
>
> I appreciate your help
>
> Eileen Dusek
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
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>
> ------------------------------
>
> Message: 8
> Date: Mon, 8 Jan 2007 16:36:06 -0500
> From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Subject: RE: [Histonet] Special stain on fixed cytology smear
> To: "eileen dusek" <eileen_dusek <@t> yahoo.com>, "histonet"
> <histonet <@t> lists.utsouthwestern.edu>
> Cc: me <e_dusek1 <@t> comcast.net>
> Message-ID:
> <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F1C <@t> sjhaexc02.sjha.org>
> Content-Type: text/plain; charset="utf-8"
>
> Yes - they work fine.
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of eileen
> dusek
> Sent: Monday, January 08, 2007 4:18 PM
> To: histonet
> Cc: me
> Subject: [Histonet] Special stain on fixed cytology smear
>
>
> Hi Everyone,
> Happiest of New Year
>
> A quick cytology question.
> Can Pneumocysytis and GMS be stained on an alcohol fixed smear?
>
> I appreciate your help
>
> Eileen Dusek
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
> Confidentiality Notice ** The information contained in this message
> may be privileged and is confidential information intended for the
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> recipient nor the employee or agent responsible for delivering this
> message to the intended recipient, you are hereby notified that any
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> reliance on the contents of this information is strictly prohibited.
> If you have received this communication in error, please notify us
> immediately by replying to the message and deleting it from your
> computer. Thank you. Saint Joseph's Health System, Inc.
>
> ------------------------------
>
> Message: 9
> Date: Mon, 8 Jan 2007 16:39:57 -0500
> From: "Douglas D Deltour" <doug <@t> ppspath.com>
> Subject: RE: [Histonet] Special stain on fixed cytology smear
> To: "'eileen dusek'" <eileen_dusek <@t> yahoo.com>, "'histonet'"
> <histonet <@t> lists.utsouthwestern.edu>
> Cc: 'me' <e_dusek1 <@t> comcast.net>
> Message-ID:
> <mailman.218.1168356722.13699.histonet <@t> lists.utsouthwestern.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Yes it can.
>
>
> Douglas D. Deltour HT(ASCP)
> Histology Supervisor
> Professional Pathology Services, PC
> One Science Court
> Suite 200
> Columbia, SC 29203
> (803)252-1913
> Fax (803)254-3262
>
> *****************************************************
> PROFESSIONAL PATHOLOGY SERVICES, PC
> NOTICE OF CONFIDENTIALITY
> This message is intended only for the use of the individual or entity to
> which it is addressed and may contain information that is privileged,
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> error, please notify me immediately.
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of eileen dusek
> Sent: Monday, January 08, 2007 4:18 PM
> To: histonet
> Cc: me
> Subject: [Histonet] Special stain on fixed cytology smear
>
> Hi Everyone,
> Happiest of New Year
>
> A quick cytology question.
> Can Pneumocysytis and GMS be stained on an alcohol fixed smear?
>
> I appreciate your help
>
> Eileen Dusek
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 8 Jan 2007 17:51:51 EST
> From: AGrobe2555 <@t> aol.com
> Subject: [Histonet] Sheep CD31
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <be1.d479922.32d42507 <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Does anyone know of an anti-CD31 that works on sheep FFPE tissues?
> Thanks,
> Albert
>
> Albert C. Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue Engineering Lab, Saint Patrick Hospital
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 8 Jan 2007 15:02:33 -0800 (PST)
> From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
> Subject: [Histonet] RUO OR ASR
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <255459.23548.qm <@t> web31304.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi All,
>
> I hate to show my stupidity, but I must be a little out of the loop
> on these. I know that RUO means research use only, but what does ASR
> mean? Don't shoot me down in flames guys!
>
> Sincerely,
>
> Akemi Allison-Tacha, BS, HT(ASCP)HTL
> President
> Phoenix Lab Consulting & Staffing
> Specializing in Histology & TMA
> Madison, WI
> E-Mail: akemiat3377 <@t> yahoo.com
> Cell: (925) 788-0900
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 8 Jan 2007 18:08:39 -0500
> From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Subject: RE: [Histonet] RUO OR ASR
> To: "Akemi Allison-Tacha" <akemiat3377 <@t> yahoo.com>,
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F24 <@t> sjhaexc02.sjha.org>
> Content-Type: text/plain; charset="utf-8"
>
> Analyte Specific Reagent - not approve by FDA...
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Akemi
> Allison-Tacha
> Sent: Monday, January 08, 2007 6:03 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RUO OR ASR
>
>
> Hi All,
>
> I hate to show my stupidity, but I must be a little out of the loop
> on these. I know that RUO means research use only, but what does ASR
> mean? Don't shoot me down in flames guys!
>
> Sincerely,
>
> Akemi Allison-Tacha, BS, HT(ASCP)HTL
> President
> Phoenix Lab Consulting & Staffing
> Specializing in Histology & TMA
> Madison, WI
> E-Mail: akemiat3377 <@t> yahoo.com
> Cell: (925) 788-0900
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message
> may be privileged and is confidential information intended for the
> use of the addressee listed above. If you are neither the intended
> recipient nor the employee or agent responsible for delivering this
> message to the intended recipient, you are hereby notified that any
> disclosure, copying, distribution or the taking of any action in
> reliance on the contents of this information is strictly prohibited.
> If you have received this communication in error, please notify us
> immediately by replying to the message and deleting it from your
> computer. Thank you. Saint Joseph's Health System, Inc.
>
> ------------------------------
>
> Message: 13
> Date: Mon, 8 Jan 2007 18:22:14 -0500
> From: "Morken, Tim" <tim.morken <@t> thermofisher.com>
> Subject: RE: [Histonet] RUO OR ASR
> To: <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <AF18F2B78D0BE84788A9938D9817383A48B3FB <@t> PGHCR-EXMB-VS-3.na.fshrnet.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Joyce, Just to clarify, when you say the ASR is " not approved by FDA"
> you make it seem they cannot be used by a diagnostic lab. But the ASR
> designation was specifically implemented to allow diagnostic labs to use
> antibodies that had not been fully validated by the company that sells
> it and puts the onus of validation on the lab that uses it. The company
> must be FDA-registered to sell ASR's and only CLIA-approved diagnostic
> labs can use ASR's as diagnostic devices. The ASR does require a notice
> that it is not FDA approved, but the lab can also attach a notice that
> ASR's do not require FDA approval.
>
>
> Tim Morken
> Lab Vision - Neomarkers
> ThermoFisher Scientific
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
> Joyce
> Sent: Monday, January 08, 2007 3:09 PM
> To: Akemi Allison-Tacha; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RUO OR ASR
>
> Analyte Specific Reagent - not approve by FDA...
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Akemi
> Allison-Tacha
> Sent: Monday, January 08, 2007 6:03 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RUO OR ASR
>
>
> Hi All,
>
> I hate to show my stupidity, but I must be a little out of the loop on
> these. I know that RUO means research use only, but what does ASR mean?
> Don't shoot me down in flames guys!
>
> Sincerely,
>
> Akemi Allison-Tacha, BS, HT(ASCP)HTL
> President
> Phoenix Lab Consulting & Staffing
> Specializing in Histology & TMA
> Madison, WI
> E-Mail: akemiat3377 <@t> yahoo.com
> Cell: (925) 788-0900
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message may
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> Saint Joseph's Health System, Inc.
>
>
>
> ------------------------------
>
> Message: 14
> Date: Mon, 8 Jan 2007 18:32:44 -0500
> From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Subject: RE: [Histonet] RUO OR ASR
> To: "Morken, Tim" <tim.morken <@t> thermofisher.com>,
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F25 <@t> sjhaexc02.sjha.org>
> Content-Type: text/plain; charset="utf-8"
>
> Thanks Tim!
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Morken,
> Tim
> Sent: Monday, January 08, 2007 6:22 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RUO OR ASR
>
>
> Joyce, Just to clarify, when you say the ASR is " not approved by FDA"
> you make it seem they cannot be used by a diagnostic lab. But the ASR
> designation was specifically implemented to allow diagnostic labs to use
> antibodies that had not been fully validated by the company that sells
> it and puts the onus of validation on the lab that uses it. The company
> must be FDA-registered to sell ASR's and only CLIA-approved diagnostic
> labs can use ASR's as diagnostic devices. The ASR does require a notice
> that it is not FDA approved, but the lab can also attach a notice that
> ASR's do not require FDA approval.
>
>
> Tim Morken
> Lab Vision - Neomarkers
> ThermoFisher Scientific
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
> Joyce
> Sent: Monday, January 08, 2007 3:09 PM
> To: Akemi Allison-Tacha; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RUO OR ASR
>
> Analyte Specific Reagent - not approve by FDA...
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Akemi
> Allison-Tacha
> Sent: Monday, January 08, 2007 6:03 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RUO OR ASR
>
>
> Hi All,
>
> I hate to show my stupidity, but I must be a little out of the loop on
> these. I know that RUO means research use only, but what does ASR mean?
> Don't shoot me down in flames guys!
>
> Sincerely,
>
> Akemi Allison-Tacha, BS, HT(ASCP)HTL
> President
> Phoenix Lab Consulting & Staffing
> Specializing in Histology & TMA
> Madison, WI
> E-Mail: akemiat3377 <@t> yahoo.com
> Cell: (925) 788-0900
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message may
> be privileged and is confidential information intended for the use of
> the addressee listed above. If you are neither the intended recipient
> nor the employee or agent responsible for delivering this message to the
> intended recipient, you are hereby notified that any disclosure,
> copying, distribution or the taking of any action in reliance on the
> contents of this information is strictly prohibited. If you have
> received this communication in error, please notify us immediately by
> replying to the message and deleting it from your computer. Thank you.
> Saint Joseph's Health System, Inc.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message
> may be privileged and is confidential information intended for the
> use of the addressee listed above. If you are neither the intended
> recipient nor the employee or agent responsible for delivering this
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> disclosure, copying, distribution or the taking of any action in
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> If you have received this communication in error, please notify us
> immediately by replying to the message and deleting it from your
> computer. Thank you. Saint Joseph's Health System, Inc.
>
> ------------------------------
>
> Message: 15
> Date: Mon, 08 Jan 2007 16:40:21 -0700
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: Re: [Histonet] Sheep CD31
> To: AGrobe2555 <@t> aol.com, Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20070108163815.01b3cd18 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Serotec veterinary antibody chart indicates CO-31D4 should work, check with
> their technical services to make sure this is true. This is their number,
> Cat # or clone ????
>
> t 03:51 PM 1/8/2007, you wrote:
>> Does anyone know of an anti-CD31 that works on sheep FFPE tissues?
>> Thanks,
>> Albert
>>
>> Albert C. Grobe, PhD
>> International Heart Institute of Montana Foundation
>> Tissue Engineering Lab, Saint Patrick Hospital
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Mon, 8 Jan 2007 22:37:21 -0800
> From: Maria Mejia <mbmphoto <@t> gmail.com>
> Subject: [Histonet] need protocol for Neu N IHC stain
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <085A3F3A-F870-4C92-93ED-DA584B575EB8 <@t> gmail.com>
> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
>
> Hello,
>
> Please, could someone provide a Neu N (neuron specific marker) IFA
> IHC protocol?
> I need to stain 40um cryostat free-floating primate fixed sections
> using this marker. I would
> welcome any suggestions from vendors too.
>
> I look forward to hearing from you.
>
> Maria Bartola Mejia
> UCSF
> Department of Neurosurgery
> San Francisco, CA
>
>
>
> ------------------------------
>
> Message: 17
> Date: Tue, 9 Jan 2007 08:08:24 -0500
> From: "LeVier, Rebecca J" <RJLevier <@t> LancasterGeneral.org>
> Subject: [Histonet] Processing with Clear-Rite 3
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2019E37C7 <@t> MAIL-LR.lha.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Please help.....If you are using Clear-Rite 3 and also processing on a
> shorter biopsy schedule, would you please share the times you are using
> at each station?
>
> Thanks in advance for all your help!
>
> Rebecca J. LeVier HT(ASCP)
> Histology Supervisor
>
> Email: rjlevier <@t> lancastergeneral.org
>
>
>
> Confidentiality Notice: This e-mail message, including any
> attachments, is for the sole use of intended recipient(s) and may
> contain confidential and privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not
> the intended recipient, please contact the sender by reply e-mail and
> destroy all copies of the original message.
>
> ------------------------------
>
> Message: 18
> Date: Tue, 09 Jan 2007 13:35:01 +0000
> From: "gloria @home" <sterntaler3 <@t> hotmail.com>
> Subject: [Histonet] Microinjection whole-mount immunolabelling
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY118-F13ED57CD22D91D55D12B07ECB30 <@t> phx.gbl>
> Content-Type: text/plain; format=flowed
>
> Hi gurus,
>
> Another question, what's the deal with this microinjection whole-mount
> immunolabelling stuff? I read in a textbook that by injecting the Ab
> directly into your embryos, you increase penetration and it works even if
> the Ab never labelled during conventional whole mount immunohistochemistry.
>
> How time-consuming, tricky is it?
>
> What are some important things I have to consider if i wish to try this out
> just in case my conventional whole-mount ihc doesn't work?
>
> I'd appreciate any help from people who've done this before!
>
> thanks
>
> _________________________________________________________________
> Get MSN Messenger emoticons and display pictures here!
> http://ilovemessenger.msn.com/?mkt=en-sg
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 9 Jan 2007 06:07:34 -0800 (PST)
> From: Jessica Piche <jessgrocki <@t> yahoo.com>
> Subject: [Histonet] B-Plus Fixative
> To: histonet <@t> lists.utsouthwestern.edu, histonet <@t> pathology.swmed.edu
> Message-ID: <701057.12724.qm <@t> web82004.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi,
>
> We are trying to get our pathologists to stop using mercury based
> fixatives for lymph node fixation. We were wondering what people's
> opinions are regarding non-mercury containing fixatives for lymph
> node fixation, particularly what you thought of B-plus fixative.
>
> Any information anyone could provide regarding any kind of lymph
> node fixative would be great!
>
> Thank you!!
>
> Jessica Piche-Grocki, HT (ASCP)
>
>
> ------------------------------
>
> Message: 20
> Date: Tue, 9 Jan 2007 06:07:34 -0800 (PST)
> From: Jessica Piche <jessgrocki <@t> yahoo.com>
> Subject: [Histonet] B-Plus Fixative
> To: histonet <@t> lists.utsouthwestern.edu, histonet <@t> pathology.swmed.edu
> Message-ID: <701057.12724.qm <@t> web82004.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi,
>
> We are trying to get our pathologists to stop using mercury based
> fixatives for lymph node fixation. We were wondering what people's
> opinions are regarding non-mercury containing fixatives for lymph
> node fixation, particularly what you thought of B-plus fixative.
>
> Any information anyone could provide regarding any kind of lymph
> node fixative would be great!
>
> Thank you!!
>
> Jessica Piche-Grocki, HT (ASCP)
>
>
> ------------------------------
>
> Message: 21
> Date: Tue, 09 Jan 2007 09:31:04 -0500
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: Re: [Histonet] B-Plus Fixative
> To: <histonet <@t> lists.utsouthwestern.edu>,
> <histonet <@t> pathology.swmed.edu>, "Jessica Piche" <jessgrocki <@t> yahoo.com>
> Message-ID: <45A360D80200007700003AAC <@t> hcnwgwds01.hh.chs>
> Content-Type: text/plain; charset=US-ASCII
>
> You are absolutely correct. No one should be using mercury-containing
> fixatives today. Several years ago, we went back to formalin for all
> lymphoid tissues and bone marrow biopsies. We have not had any problems
> as long as the specimen is fixed properly.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>>>> Jessica Piche <jessgrocki <@t> yahoo.com> 01/09/07 9:07 AM >>>
> Hi,
>
> We are trying to get our pathologists to stop using mercury based
> fixatives for lymph node fixation. We were wondering what people's
> opinions are regarding non-mercury containing fixatives for lymph node
> fixation, particularly what you thought of B-plus fixative.
>
> Any information anyone could provide regarding any kind of lymph node
> fixative would be great!
>
> Thank you!!
>
> Jessica Piche-Grocki, HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Confidentiality Notice
>
> This e-mail message, including any attachments, is for the sole use of
> the intended recipient(s) and may contain confidential or proprietary
> information which is legally privileged. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please promptly contact the sender by reply e-mail and
> destroy all copies of the original message.
>
>
>
> ------------------------------
>
> Message: 22
> Date: Tue, 09 Jan 2007 09:31:04 -0500
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: Re: [Histonet] B-Plus Fixative
> To: <histonet <@t> lists.utsouthwestern.edu>,
> <histonet <@t> pathology.swmed.edu>, "Jessica Piche" <jessgrocki <@t> yahoo.com>
> Message-ID: <45A360D80200007700003AAC <@t> hcnwgwds01.hh.chs>
> Content-Type: text/plain; charset=US-ASCII
>
> You are absolutely correct. No one should be using mercury-containing
> fixatives today. Several years ago, we went back to formalin for all
> lymphoid tissues and bone marrow biopsies. We have not had any problems
> as long as the specimen is fixed properly.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>>>> Jessica Piche <jessgrocki <@t> yahoo.com> 01/09/07 9:07 AM >>>
> Hi,
>
> We are trying to get our pathologists to stop using mercury based
> fixatives for lymph node fixation. We were wondering what people's
> opinions are regarding non-mercury containing fixatives for lymph node
> fixation, particularly what you thought of B-plus fixative.
>
> Any information anyone could provide regarding any kind of lymph node
> fixative would be great!
>
> Thank you!!
>
> Jessica Piche-Grocki, HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Confidentiality Notice
>
> This e-mail message, including any attachments, is for the sole use of
> the intended recipient(s) and may contain confidential or proprietary
> information which is legally privileged. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please promptly contact the sender by reply e-mail and
> destroy all copies of the original message.
>
>
>
> ------------------------------
>
> Message: 23
> Date: Tue, 9 Jan 2007 14:58:04 -0000
> From: Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
> Subject: [Histonet] RE: CD 10 protocol
> To: "Histonet submissions (histonet <@t> lists.utsouthwestern.edu)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <F2D584187C727B4CBFE008435881E8970234BD5E <@t> rlixch>
> Content-Type: text/plain
>
> Hi
> We have a Ventana Benchmark XT and use Vector's CD 10 (clone 56C6) at 1/50
> with the protocol standard CC1 primary incubation for 56 minutes at 37C and
> no amplification.
>
> Jacqui
>
>
>
> DISCLAIMER: This e-mail is confidential and privileged. If you are not the
> intended recipient please accept our apologies; please do not disclose, copy
> or distribute information in this e-mail or take any action in reliance on
> its contents: to do so is strictly prohibited and may be unlawful. Please
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> deleting it. Comments or opinions expressed in this email are those of
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> views of the Trust, its management or employees. University Hospitals of
> Morecambe Bay NHS Trust is not responsible and disclaims any and all
> liability for the content of comments written within.Thank you for your
> co-operation.
>
>
>
>
> ------------------------------
>
> Message: 24
> Date: Tue, 9 Jan 2007 07:07:19 -0800 (PST)
> From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
> Subject: [Histonet] RUO or ASR
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <134014.25099.qm <@t> web31301.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi Tim,
>
> You made my day! Thanks for the information. You gave me exactly
> what I wanted. I have been focusing on development of other
> products & setting-up TMA labs in the past few years. I have not had
> as intimate of a relationship with IHC as I did several years ago.
> Marcia Welch wanted me to propose the question to the histonet since
> she is not a subscriber.
>
> Best Regards,
>
> Akemi Allison-Tacha, BS, HT(ASCP)HTL
> President
> Phoenix Lab Consulting & Staffing
> Specializing in Histology & TMA
> Madison, WI
> E-Mail: akemiat3377 <@t> yahoo.com
> Cell: (925) 788-0900
>
>
>
> ------------------------------
>
> Message: 25
> Date: Tue, 09 Jan 2007 08:22:07 -0700
> From: Andrea Grantham <algranth <@t> u.arizona.edu>
> Subject: [Histonet] Hooray for Histotechs! (have to brag just a
> little)
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <4.3.2.7.2.20070109080945.00da0d58 <@t> algranth.inbox.email.arizona.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> I just had to pass this one along - we are so excited!
> My student, Kristin Sweetser, has just found out that she was awarded one
> of the ASCP student scholarships. Kristin is a student in our histology
> program at Pima Community College and is just about to complete her co-op
> in my lab. She is an exemplary student and already a very good histotech.
> We are excited for her and also for the program at PCC which is just a few
> years old.
>
> (If you want to send congrats to Kristin you can send them to this address
> and I will forward them to her.)
>
> Thanks for letting me brag just a little.
>
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
>
>
> ------------------------------
>
> Message: 26
> Date: Tue, 9 Jan 2007 07:26:05 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Special stain on fixed cytology smear
> To: eileen dusek <eileen_dusek <@t> yahoo.com>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Cc: me <e_dusek1 <@t> comcast.net>
> Message-ID: <20070109152605.55010.qmail <@t> web61219.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Yes, they can!
> Usually the GMS is used to detect the Pneumocystis and even IHC
> procedures can be done in alcohol fixed smears, and in this case they
> do not require HIER.
> René J.
>
> eileen dusek <eileen_dusek <@t> yahoo.com> wrote:
> Hi Everyone,
> Happiest of New Year
>
> A quick cytology question.
> Can Pneumocysytis and GMS be stained on an alcohol fixed smear?
>
> I appreciate your help
>
> Eileen Dusek
>
> __________________________________________________
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>
>
>
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>
> ------------------------------
>
> Message: 27
> Date: Tue, 9 Jan 2007 07:31:54 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] B-Plus Fixative
> To: Jessica Piche <jessgrocki <@t> yahoo.com>,
> histonet <@t> lists.utsouthwestern.edu, histonet <@t> pathology.swmed.edu
> Message-ID: <737016.79654.qm <@t> web61214.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Jessica:
> Mercury containing fixatives are a thing of the past; they are
> personally and environmentally dangerous and, although nuclear detail
> is very good, you can obtain similar details following very simple
> steps:
> 1-make sure your fixative is REALLY neutral, the fresher, the better;
> 2-fix the biopsies correctly;
> 3-section as thin as possible;
> 4-make sure that the sections are TOTALLY drained before heat drying
> them; and
> 5-use the regressive hematoxylin with acetic acid.
> René J.
>
> Jessica Piche <jessgrocki <@t> yahoo.com> wrote:
> Hi,
>
> We are trying to get our pathologists to stop using mercury based
> fixatives for lymph node fixation. We were wondering what people's
> opinions are regarding non-mercury containing fixatives for lymph
> node fixation, particularly what you thought of B-plus fixative.
>
> Any information anyone could provide regarding any kind of lymph node
> fixative would be great!
>
> Thank you!!
>
> Jessica Piche-Grocki, HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 38, Issue 10
> ****************************************
>
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cumming School of Veterinary Medicine
200 Westborough Road
North Grafton, MA 01536
Tel:508-839-5395
Fax:508-839-7922
email: melissa.mazan <@t> tufts.edu
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