[Histonet] Perfusion fixation of pig lungs

[Kevin Gibbon]

 I used to fix whole human and rat lungs by removing them from the body
and inflating via the trachea, then tie the trachea shut. The fixation
is excellent for lung tissue.
kevin

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles
Scouten
Sent: Friday, January 05, 2007 11:52 AM
To: LNLJ (Lene Lyngsie Jensen); histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Perfusion fixation of pig lungs

The fastest (less advance surgery) and most effective perfusion would be
whole body under higher than physiological pressure.  See the discussion
in Microscopy Today, May 2006 issue, for some ideas.  You would need
about 5 gallons of liquid prewash, and 2 or 3 gallons of fixative, for a
quess.  

http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=4
71005&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=S
acrifice+Equipment&idsubcategory=21

See the link above for ideas of what is needed, but our fluid tanks are
not large enough.  You (or we) would rig something similar on some
Carboy Tanks.

Getting through the heart and applying fluid at 300 mm Hg, the pig will
be drained of blood in seconds.  Fixative can then be applied and will
get there faster, measured from the time the diaphragm or chest cavity
is punctured, than any other way I know of.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com
5918 Evergreen Blvd. 
St. Louis, MO 63134
Ph: 314 522 0300 x 342
FAX  314 522 0377
cwscouten <@t> myneurolab.com
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LNLJ
(Lene Lyngsie Jensen)
Sent: Thursday, January 04, 2007 4:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Perfusion fixation of pig lungs

Hey.

 

I hope that someone here can help me.

 

We are planning to do some research in pig lungs. Due to this
investigation it is very important to have a fast and effective
fixation.

 

We will therefore try to make a perfusion fixation. But a pig is a very
large animal and therefore we would like to limit the fixation only to
the lung region, and not a full body perfusion fixation.

 

Is there anyone how has experience with perfusion fixation limit to only
one organ in pigs? 

 

All suggestion will be read with enthusiasm.

 

Experience from similar fixation methods in other animals is welcome as
well.

 

Thanks.

 

 

________________________________________________

Lene Lyngsie Jensen



 

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