[Histonet] ISH protocol with DIG-labeled probes

Andrea Hooper anh2006 <@t> med.cornell.edu
Wed Jan 3 17:51:35 CST 2007

Dear All,

I was wondering if someone has a tried and true ISH protocol (I know, 
a stretch!) which they could send to me for paraffin sections using 
DIG labeled probes? I have tried some homemade mix-and-match 
protocols and am at my wits end getting the specificity to be 
accurate. I know my probes are good as I use them for other purposes 
with good success so that isn't it.


Thank you in advance,

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