[Histonet] Secondary antibody for Ventana detection kits

Patsy Ruegg pruegg <@t> ihctech.net
Wed Feb 28 16:14:57 CST 2007

Can't help you with Ventana application but I did want to mention that
BioCare now sells an anti-goat labeled polymer hrp detection system that
works well.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda
Sent: Wednesday, February 28, 2007 2:09 PM
To: Histonet
Subject: [Histonet] Secondary antibody for Ventana detection kits


I have the need to optimize a goat anti-human antibody on the Ventana XT
immunostainer.  In order to incorporate an anti-goat secondary I am
using their "open secondary" protocol with an iView DAB detection kit.
I am wondering whose antibody people are using in these circumstances.
Ventana says that people have used Jackson ImmunoResearch's biotinylated
R x G F(ab')2 (cat. #: 305-066-003) for this purpose with success.  I
would like to actually talk to anyone who has done this as I have
concerns about their storage requirements and putting this antibody in a

Thank you in advance for your help,

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

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