[Histonet] Secondary antibody for Ventana detection kits

Sebree Linda A. LSebree <@t> uwhealth.org
Wed Feb 28 15:08:36 CST 2007


I have the need to optimize a goat anti-human antibody on the Ventana XT
immunostainer.  In order to incorporate an anti-goat secondary I am
using their "open secondary" protocol with an iView DAB detection kit.
I am wondering whose antibody people are using in these circumstances.
Ventana says that people have used Jackson ImmunoResearch's biotinylated
R x G F(ab')2 (cat. #: 305-066-003) for this purpose with success.  I
would like to actually talk to anyone who has done this as I have
concerns about their storage requirements and putting this antibody in a

Thank you in advance for your help,

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

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