[Histonet] RE: microtome problems

Tue Feb 27 14:18:05 CST 2007

I had the same problem, and we had our Leica microtome serviced- the block holder was not holding the blocks tight enough, and when the service tech replaced it, problem solved.
Tiffany
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Today's Topics:

   1. Microtome problems (mkaulahao <@t> bellsouth.net)
   2. Re: Microtome problems (Dawn Cowie)
   3. Re: Microtome problems (Rene J Buesa)

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Message: 1
Date: Sun, 25 Feb 2007 6:24:57 -0500
From: <mkaulahao <@t> bellsouth.net>
Subject: [Histonet] Microtome problems
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<20070225112457.GPR12624.ibm64aec.bellsouth.net <@t> mail.bellsouth.net>
Content-Type: text/plain; charset=ISO-8859-1

Hi everyone,
This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick?
Mary Beth

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Message: 2
Date: Sun, 25 Feb 2007 05:32:06 -0800 (PST)
From: Dawn Cowie <dlcowie <@t> prodigy.net>
Subject: Re: [Histonet] Microtome problems
To: mkaulahao <@t> bellsouth.net, histonet <@t> lists.utsouthwestern.edu
Message-ID: <748960.32009.qm <@t> web81001.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

hi Mary Beth,
  If you think humidity may be the problem, try placing a piece of warm damp gauze on either side of the microtome (as close to the blade holder as possible). This has worked for me sometimes. You can also try spraying static guard around the blade holder area.

  Dawn L. Cowie, HT (ASCP)
  Histology Supervisor
  Pensacola Pathologists, PA
  Pensacola, FL

mkaulahao <@t> bellsouth.net wrote:
  Hi everyone,
This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick?
Mary Beth

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Message: 3
Date: Sun, 25 Feb 2007 07:19:26 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Microtome problems
To: mkaulahao <@t> bellsouth.net, histonet <@t> lists.utsouthwestern.edu
Message-ID: <20070225151926.72418.qmail <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

How many times your Leitz microtome has been serviced since 1994? I think you should have it serviced (overhawlled) before starting to worry about humidity. If the microtome has some adjustment problems they will be more evident in large blocks than in smaller ones (biopsies) due to less resistance in the smaller ones.
  Also think that if you are in a new lab your microtome had to be MOVED from the old one, and some disadjustments may hace occurred while moving to the new lab.
  Have it serviced!
  René J.

mkaulahao <@t> bellsouth.net wrote:
  Hi everyone,
This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick?
Mary Beth

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End of Histonet Digest, Vol 39, Issue 41
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