[Histonet] Dispensable knife

[Jane M Flinn]

What type of disposable blades for a cryostat would you recommend? We are slicing mouse brains. Leica high profile have been suggested.

jane






Dr. Jane Flinn
Director, Biopsychology Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA  22030
Phone: 703-993-4107
Fax: 703-993-1359


----- Original Message -----
From: Cheryl Kerry <tkngflght <@t> yahoo.com>
Date: Tuesday, February 27, 2007 12:58 pm
Subject: RE: [Histonet] Dispensable knife

> While I don't have a list of other blade brands for you-- I do 
> have a few
> things to add to your list when considering cost of blades in general.
> 
> 
> 
> Have you calculated how many slides or blocks your techs produce 
> per box of
> blades?  AccuEdge blades do seem to be pricey but I've always gone 
> throughmore of most of the other kinds...price per slide was 
> uniformly better with
> low-profile AccuEdge and a thicker one like Sturkey for bone and hard
> tissue.
> 
> 
> 
> Also consider the frustration factor.  I don't care HOW much the 
> blades cost
> when I'm trying to get 40 slides off a hair-thin prostate biopsy!  In
> fairness, it takes a couple of days with a new blade to get used 
> to the
> differences.  Having both types around during a new blade trial 
> reduces the
> fear factor and keeps people's minds a little more receptive to 
> the changes.
> 
> 
> 
> Some labs stock three or four different types--each tech picks 
> what works
> for them and is responsible to keep the order clerk informed of 
> when they
> need more.
> 
> 
> 
> My two cents :-)
> 
> 
> 
> Cheryl
> 
> 
> 
> Cheryl Kerry, HT(ASCP)
> 
> Full Staff Inc.
> 
> 281.852.9457
> 
> 800.756.3309
> 
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> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Marcia Funk
> Sent: Tuesday, February 27, 2007 11:30 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Dispensable knife
> 
> 
> 
> Howdy,
> 
> 
> 
> I would like to look at different microtome blades.  We are using 
> Accu-Edge
> Low Profile and they
> 
> seem a bit pricy.  I would like info on what types your lab is 
> using .
> 
> 
> 
> 
> 
> 
> 
> 
> 
> 
> 
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