[Histonet] Stain for...

[Andrea Grantham]

There is a stain protocol for pectin in Ruzin's book, Plant Microtechnique 
and Microscopy, page 152. The protocol indicates that it can be used for 
paraffin sections. There are also stains for lignin (I've done these) in 
the book as well. For cellulose try a PAS (Ruzin, page 148).

Andi Grantham



At 06:09 PM 2/21/2007 -0500, mgolbaba <@t> uoguelph.ca wrote:

>Hello everyone,
>
>I am looking for a specific stain to observe the cellulose (fibers),
>hemicellulose, and pectin separately in my plant specimens which are
>dried and ground wheat straw.
>I greatly appreciate if you let me know your recommended stains and
>their protocols.
>Actually, I have found several stains for lignin (e.g. phloroglucinol)
>and pectin not cellulose and hemicellulose but, unfortunately, they
>were for live tissues.
>Many thanks in advance.
>
>Best Regards,
>Mahsa
>
>-----------------------------
>Mahsa Golbabaie
>MSc Student,
>Department of Plant Agriculture, University of Guelph, Canada
>Department of Chemical Engineering, University of Waterloo, Canada
>Email: mgolbaba <@t> uoguelph.ca
>mgolbaba <@t> engmail.uwaterloo.ca
>
>
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html