[Histonet] HIF-1alpha immunohistochemistry in FFPE in mouse

[PALMER Jason (SVHM)]

Hi.

We are looking for an antibody that will label HIF-1alpha in paraffin
sections in mouse tissue. Would prefer not to do mouse on mouse. There
are 2 goat polyclonals I found in a search, from Santa Cruz - prodcuts
sc-8711 and sc-12542. The data sheets don't mention any use with
immuno's on FFPE, but a paper I recently came across uses one of them
(can't be sure which one yet...) on FFPE sections of mouse pancreatic
islets. Also in my searching I found a Chemicon rabbit polyclonal -
AB3883 - which they claim has been used with immuno's on FFPE sections.
Has anybody had success with any of these products? Any feedback greatly
appreciated.

We did try a Novus Biologicals monoclonal (NB 100-123) on rat tissue a
few years back with little success. My impression is that HIF1-a
immunostaining generally speaking is not easy to achieve...

Thanks,
Jason

Jason Palmer
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.palmer <@t> svhm.org.au






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