[Histonet] HELP!!! vertical IHC staining artifact and steamer retrival

shawnster73 <@t> aol.com shawnster73 <@t> aol.com
Mon Feb 12 13:47:50 CST 2007

 I'm hoping that you will be able to help me with my staining problem. I am using a vector ABC kit and staining rat lung with ULEX stain. I am using a steamer for 50 minutes for retrival before staining. I retriving in staining dishes that hold 20 slides. I have tried just putting one dish into the steamer and tried puttng 2 dishes into the steamer at a time and still get the same results. I have also tried using the same staining dishes in the same steamer, using the same protocol, but only stained 2 slides and the staining came out beautiful. I have had suggestions of everything from the pap pen caused the problem to the retrival caused it. I am suspicious of the steamer. If you were using a rack in the steamer, is there a distace that you would keep between the slides to ensure proper solution movement so that the slides are not cooked? Any suggestions at all that might help solve my problem would be greatly appreciated. 
Check out the new AOL.  Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more.

More information about the Histonet mailing list