[Histonet] Prolong gold tips

[Thomas Pier]

I've used Prolong Gold before.  Typically, I put it on the slide,
coverslip, turn the slide upside down on a wipe all, press down on the
slide to remove excess, then put the slide in a 37 degree oven for about
a half an hour.  You might also try SlowFade Gold w/ DAPI.

Tom Pier

>>> "Till, Renee" <TillRenee <@t> uams.edu> 02/12/07 10:08 AM >>>
Quite a few people seem to use this so I thought I'd try it and see if
there
is any difference from Vectashield. I have tried the versions of both
that
have DAPI. Couple of questions about the prolong. How long do you
usually let
it cure before looking at the slides? How long before you seal the
slides?
Also, it says to remove the excess moisture. How picky is it on this?
The
first few slides I tried were not optimally done, but still it did not
seem
as good as some of my slides with Vectashield. The DAPI was less bright
and
the intensity varied in areas. I wondered if this was because I might
have
got parts of the tissue drier than others (I blotted with a kimwipe). 

 

Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72202

Office (501)364-2785

Fax (501)364-3161

 

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