sectioning Re: [Histonet] Animal Tissue
gcallis <@t> montana.edu
Mon Feb 12 09:59:25 CST 2007
Animal tissue is very lean, and you probably are over dehydrating or drying
out the tissue during processing. Our mouse tissues are on a much shorter
processing schedule than we ever used for human tissues (which tend to be a
bit more fatty). Do not add heat to processing schedule (solvents), and
cut back on times in all solvents.
The smaller the tissue i.e. lymph nodes and slices of brain is on a much
shorter schedule here.
You paraffin temperatures should be at 60C or less.
At 08:57 PM 2/9/2007, you wrote:
>I need to get some input on cutting animal tissue. I have always worked in a
>clinical setting and have recently moved into a research lab. I find that the
> tissue is very brittle and needs to be iced for an extremely amount of
>Can any of you animal cutting histo techs please give me some advice on what
>type of paraffin you are using and what processing schedule you are using. I
>have recently changed to Richard Allen type 9 paraffin and it seems to have
>helped some. I would appreciate any advice you can give me.
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