[Histonet] Softening animal tissue
Pam V
pamvlies <@t> sbcglobal.net
Sun Feb 11 18:46:02 CST 2007
Hi all...
One of the things I've done for years is soak tissue with a dilute glycerin. Not exactly sure why, but the cells do not blow up, nor is the staining affected, neither routine nor immunos..Sections adhere to the slides just fine.
I'm at Evanston Northwestern Hospital in Evanston Illinois...we do some work on animal tissue also and this has worked well for me. It also works on decals, and very well on blood, such as bone marrow clots, as well as lymph nodes. It also allows thinner sectioning.
I haven't done any recent searches for the use of glycerin and some people are hesitant, but I did see that it's suggested by Frieda Carson and is a question in the ASCP Board of Registry practice exam text.
Pam Vlies HTASCP
ENH Histology lab..
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Subject: Histonet Digest, Vol 39, Issue 18
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Today's Topics:
1. Re: Animal Tissue (Esther Peters)
2. Re: Animal Tissue (Amos Brooks)
3. Re: Iron Control (Jennifer MacDonald)
4. Job opening (raj)
5. GPR58 antibody (Ana MERINO-TRIGO)
----------------------------------------------------------------------
Message: 1
Date: Sat, 10 Feb 2007 14:43:28 -0500
From: Esther Peters <esther.peters <@t> verizon.net>
Subject: Re: [Histonet] Animal Tissue
To: jhnspam <@t> aol.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <45CE2060.70203 <@t> verizon.net>
Content-Type: text/plain; charset=us-ascii; format=flowed
Pam,
What animal tissue are you using? I recall learning from HistoNet a
while back that rodent tissues should be processed for shorter periods
(30 min. per solution and paraffin change compared to 60 min.) because
of potential hardening issues. This is also noted in several mouse/rat
or small animal processing schedules provided in the Animal Processing
Manual published by the National Society for Histotechnology's
Veterinary, Industry and Research Committee, edited by Gayle Callis and
Diane Sterchi (along with many other helpful tips for non-human tissue
handling!). I think it (or an updated version) is still available from
NSH (Gayle, Diane?).
Esther Peters, Ph.D.
George Mason University
jhnspam <@t> aol.com wrote:
> I need to get some input on cutting animal tissue. I have always worked in a
> clinical setting and have recently moved into a research lab. I find that the
> tissue is very brittle and needs to be iced for an extremely amount of time.
> Can any of you animal cutting histo techs please give me some advice on what
> type of paraffin you are using and what processing schedule you are using. I
> have recently changed to Richard Allen type 9 paraffin and it seems to have
> helped some. I would appreciate any advice you can give me.
>
> Thanks,
> Pam
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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>
------------------------------
Message: 2
Date: Sat, 10 Feb 2007 17:22:48 -0500
From: "Amos Brooks" <amosbrooks <@t> gmail.com>
Subject: Re: [Histonet] Animal Tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<582736990702101422u355b9e06rd0c40e0c575e8e97 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hi,
If anyone has any replies to this please do it to the list (or include
me as well) as I seem to be in a similar boat having recently accepted a
transfer (within the same company) to a research lab. I must admit to some
trepidation about animal tissue not having much experience with it. My
experiences are primarily clinical. I've heard some stories of great
frustration about animal tx. I'm sure I can handle it but there is always a
learning curve.
Thanks
Amos Brooks
Message: 19
Date: Fri, 9 Feb 2007 22:57:36 EST
From: jhnspam <@t> aol.com
Subject: [Histonet] Animal Tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <c65.c5cc97a.32fe9cb0 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I need to get some input on cutting animal tissue. I have always worked in
a
clinical setting and have recently moved into a research lab. I find that
the
tissue is very brittle and needs to be iced for an extremely amount of
time.
Can any of you animal cutting histo techs please give me some advice on
what
type of paraffin you are using and what processing schedule you are using.
I
have recently changed to Richard Allen type 9 paraffin and it seems to have
helped some. I would appreciate any advice you can give me.
Thanks,
Pam
------------------------------
Message: 3
Date: Sat, 10 Feb 2007 17:49:46 -0800
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Iron Control
To: "sheila adey" <sheila_adey <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF18F6358F.443EAEEB-ON8825727F.000A0CF8-8825727F.000A0CFA <@t> mtsac.edu>
Content-Type: text/plain; charset="UTF-8"
Spleen makes for a sensitive iron control. The amount of ir sufficient, but not overwhelming.
Jennifer
-----histonet-bounces <@t> lists.utsou
To: histonet <@t> lists.utsouthwestern.edu
From: "sheila Sent by: histonet-bounces <@t> lists. Date: 02/10/2007 07:18AM
Subject: [Histonet] Iron Hello All,
We are almost out of Iron Control. We h chronic
hemosiderosis condition. The Docs sa before we
find a suitable control. Could any that might
be easier to find?
Th Sheila Adey HT MLT
Port Huron Hospital
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------------------------------
Message: 4
Date: Sat, 10 Feb 2007 21:58:51 -0500
From: raj <raj <@t> bluemarble.net>
Subject: [Histonet] Job opening
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <45CE866B.3B47CBA9 <@t> bluemarble.net>
Content-Type: text/plain; charset=us-ascii
We have a opening at Bloomington Hospital for a reg. HT. Bloomington,
IN. It is a day shift job. Bloomington is about 50 miles south of
Indianapolis,IN. It the home of Indiana University. If interested
please reply and I will direct you to the HR dept.
Thank You
Rebecca A. Johnson
------------------------------
Message: 5
Date: Sun, 11 Feb 2007 17:09:40 +0100 (CET)
From: Ana MERINO-TRIGO <ana.merino-trigo <@t> wanadoo.fr>
Subject: [Histonet] GPR58 antibody
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <24357752.403521171210180349.JavaMail.www <@t> wwinf1603>
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Hello Histonet,
I was wondering if anyone has experience with GPR58, G protein coupled receptor (alias TAAR2, trace amine associated receptor 2) human antibody from LifeSpan using Ventana. So far, I've had no luck on paraffin sections. I will need to test the expression on human pancreas (paraffin sections). I'm using human cerebelum as positive control, as it's described in the literature to be expressed in this tissue.
I've tested, CC1, CC2 and protease using DAB kit for the developing. With CC2 I do lose mostly of my tissues, no sure if is a buffer batch problem or if conditions are really strong for cerebellum tissue.
Any advice it will be really appreciate it.
thanks a lot,
Ana
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