[Histonet] Mouse blood chemistry analysis

Fri Feb 9 12:27:14 CST 2007

Histonet friends,

We have a Endocheck plus blood chemistry analyzer in our laboratory. In one 
of our project, we need to use to analyze mouse plasma samples. We have 
problems to get consistant results. And the reproducibility is poor with 
large variation. I am not sure whether someone has experience with this 
machine. Is there any better blood chemistry analyzer for mous plasma 
analysis? We have limited sample volume. Anyone want to share some 
experience? Thanks.

===========================
Ze Lu, Ph.D.
Optimum Therapeutics, LLC

----- Original Message ----- 
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, February 09, 2007 12:59 PM
Subject: Histonet Digest, Vol 39, Issue 15

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> Today's Topics:
>
>   1. formalin fixed mouse ears-help (Gayle Callis)
>   2. smooth muscle cell actin for rabbit  (Galina Deyneko)
>   3. 2007 Region III meeting in NC (Bly Haverland)
>   4. AFA fixative (Cristina Nunes)
>   5. Re:Rabbit Smooth Muscle actin (AGrobe2555 <@t> aol.com)
>   6. Re: AFA fixative (Geoff McAuliffe)
>   7. Techs & Transcriptionists (McCord, Cherie)
>   8. GSH 2007  meeting REMINDER (Shirley Powell)
>   9. table for microscope (wen eng)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 09 Feb 2007 08:20:13 -0700
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: [Histonet] formalin fixed mouse ears-help
> To: "Mary Beauchamp" <mbeaucha <@t> benaroyaresearch.org>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20070209080907.01b28190 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Mary,
>
> Since you did not say what your processing schedule is for these ears, I
> suspect infiltration in paraffin is not very good so extend the time there
> and use a vacuum.  This still denser tissue even though it appears very
> thin, and those layers can be a problem.   Try at least three changes 
> under
> vacuum.  Hopefully you have vacuum and pressure on your processor, and
> don't add heat to the processing solvents, these are skinny dry tissues to
> begin with.
>
> 4. Lower the temperature of the water bath and don't over soak a trimmed
> block on ice water.
>
> Nothing is silly or simple if you are having problems.  Good luck
>
>>I'm having problems with 10% neutral buffered formalin fixed mouse ears
>>embedded in paraffin. They are splitting from the cartilage as soon, and
>>I mean, as soon as they hit the water bath.
>>
>>I'm open to any suggestions, no matter how silly or simple. This is a
>>chronic problem and these mouse ears will continue to be submitted to me
>>for a long time.
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 9 Feb 2007 07:51:32 -0800 (PST)
> From: Galina Deyneko <galinadeyneko <@t> yahoo.com>
> Subject: [Histonet] smooth muscle cell actin for rabbit
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <842576.99403.qm <@t> web33114.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Dear Colleagues,
>  I am looking for primary antibody against smooth muscle cell actin in 
> FFPE  rabbit aorta  with athero lesion. I have used the mouse monoclonal 
> primary AB from Biocare #CM001, clone 1A4, reacts with human ,rat, rabbit 
> etc. with following protocol:
>  No pre-treatment.
>  Peroxidase blocking
>  Protein blocking
>  Incubation with AB 2 hours at RT
>  Detection with MACH 2 polymer -HRP (Biocare)
>  Chromogen: DAB.
>  AB gives non-specific staining in macrophages, and foam cells, and SMC 
> are stained light brown.
>  With mouse aorta and sinus with MOM kit this AB works very well.
>  The same results with monkey's coronary arteries.
>  Any information and protocols for both species are highly appreciated.
>  Sincerely.
>  Galina Deyneko
>  CardioVasccular Department
>  Novartis, Cambridge, MA
>  617-871-7613.
>
>
>
>
>
> ---------------------------------
> Bored stiff? Loosen up...
> Download and play hundreds of games for free on Yahoo! Games.
>
> ------------------------------
>
> Message: 3
> Date: Fri, 09 Feb 2007 11:26:49 -0500
> From: "Bly Haverland" <corycody <@t> msn.com>
> Subject: [Histonet] 2007 Region III meeting in NC
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY106-F43D33D2005B650B04C94CD39C0 <@t> phx.gbl>
> Content-Type: text/plain; format=flowed
>
> The North Carolina Society of Histopathology Technologists is pleased to
> announce it will be hosting the 2007 Region III meeting this year taking
> place March 22-24, 2007 in Research Triangle Park, North Carolina at the
> Raleigh-Durham Airport Hilton. The Society invites anyone interested in
> histology to attend. Membership is not required for participation. The
> officers and volunteers of NCSHT have put together an exciting program 
> this
> year, and they look forward to as many participants as possible. A 
> complete
> listing of the Region III program along with registration forms is posted 
> on
> the NSH website at www.nsh.org
>
> Thank you.
> Bly Haverland HT, HTL
> Triangle Histology Services, Inc.
> corycody <@t> msn.com
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 9 Feb 2007 16:27:09 -0000
> From: "Cristina Nunes" <cnunes <@t> ipimar.pt>
> Subject: [Histonet] AFA fixative
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <200702091631.l19GVtPK002913 <@t> neptuno.ipimar.pt>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear histoneters,
> For several years, in the Institute where I am working, the main fixative
> used for the preservation of gonads of fish was AFA, with apparently good
> results. Then, at a given moment (before I arrived to the Institute), it 
> was
> decided to change from AFA to a formalin solution because of the higher
> toxicity of the former. The histological results were slightly less good 
> but
> for practical reasons (most fish tissue fixations occur at sea, on board 
> the
> research vessels), it was considered to be the best solution. However,
> nobody was able to explain me why AFA is much more toxic than a formalin
> solution (I am sorry for my ignorance in terms of chemistry). Could anyone
> clarify me on that point?
> Many thanks in advance and a very nice week-end.
> Regards,
> Cristina Nunes.
>
>><<<>
> ......................................................................><<<>
> Cristina De Amaral P. Nunes
> INIAP-IPIMAR Fisheries and Sea Research Institute
> Marine Resources Department
> Avenida Brasília
> 1449-006 Lisboa
> Portugal
> Tel: + 351 21 302 71 55
> Fax: + 351 21 301 59 48
> Email: cnunes <@t> ipimar.pt
>><<<>
> .....................................................................><<<>
>
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 9 Feb 2007 11:36:41 EST
> From: AGrobe2555 <@t> aol.com
> Subject: [Histonet] Re:Rabbit Smooth Muscle actin
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <d12.642a75c.32fdfd19 <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> I've used the monoclonal anti-smooth muscle cell actin (A2547) from SIGMA
> with great success in rabbit tissues fixed in Histochoice.  Might work for 
> you.
> Albert
>
> Albert C.  Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue  Engineering Lab, Saint Patrick  Hospital
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 09 Feb 2007 11:43:22 -0500
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] AFA fixative
> To: Cristina Nunes <cnunes <@t> ipimar.pt>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <45CCA4AA.3000402 <@t> umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> Hi Christina.
>
>    Well, AFA is alcohol + formaldehyde + acetic acid, none of these are
> good for you but neither is buffered formalin. That said, the alcohol in
> AFA will "carry" the other components across skin by acting as a solvent
> to the lipids in skin. Aqueous fixed like buffered formalin are much
> less likely to do this. So the good penetration properties of AFA could
> render it more toxic. Gloves would solve the problem.
>
> Geoff
>
> Cristina Nunes wrote:
>
>>Dear histoneters,
>>For several years, in the Institute where I am working, the main fixative
>>used for the preservation of gonads of fish was AFA, with apparently good
>>results. Then, at a given moment (before I arrived to the Institute), it 
>>was
>>decided to change from AFA to a formalin solution because of the higher
>>toxicity of the former. The histological results were slightly less good 
>>but
>>for practical reasons (most fish tissue fixations occur at sea, on board 
>>the
>>research vessels), it was considered to be the best solution. However,
>>nobody was able to explain me why AFA is much more toxic than a formalin
>>solution (I am sorry for my ignorance in terms of chemistry). Could anyone
>>clarify me on that point?
>>Many thanks in advance and a very nice week-end.
>>Regards,
>>Cristina Nunes.
>>
>>
>>
>>><<<>
>>>
>>>
>>......................................................................><<<>
>>Cristina De Amaral P. Nunes
>>INIAP-IPIMAR Fisheries and Sea Research Institute
>>Marine Resources Department
>>Avenida Brasília
>>1449-006 Lisboa
>>Portugal
>>Tel: + 351 21 302 71 55
>>Fax: + 351 21 301 59 48
>>Email: cnunes <@t> ipimar.pt
>>
>>
>>><<<>
>>>
>>>
>>.....................................................................><<<>
>>
>>
>>
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>>
>
>
> -- 
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 9 Feb 2007 11:07:27 -0600
> From: "McCord, Cherie" <cmccord <@t> ameripath.com>
> Subject: [Histonet] Techs & Transcriptionists
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <E5E86C7298523E4480146C3DB7B3B63F02D3A212 <@t> TXCLMAIL01.ameripath.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Anyone know of a Tech who would be interested in working in the Atlanta
> area (Marietta, GA) to be exact.  The hours are 10pm - 6:30am.  Right
> now you would be the only person.  Grossing, cutting, staining and IHC
> on GI biopsies.  We are also in desperate need of 2 transcriptionists.
> Any contacts would be greatly appreciated.
>
>
>
> Denise McCord
>
> Lab Manager
>
> DermPath Diagnostics
>
> (A division of AmeriPath, Inc.)
>
> Marietta, GA  30067
>
> cmccord <@t> AmeriPath.com
>
> 770-612-1395 ext. 212
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 09 Feb 2007 12:27:01 -0500
> From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
> Subject: [Histonet] GSH 2007  meeting REMINDER
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <01MCXFGX2OC48WWK57 <@t> Macon2.Mercer.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi Friends,
>
> Yes I am shouting.  I am again posting the program information for our
> meeting.  The program in .PDF format can be found on our new website
> www.histosearch.com/gsh.  Click on GSH Symposium and the program can be
> downloaded from there.
>
> ****Please note, the deadline for registration at Callaway Garden Mountain
> Creek Inn  is March 1st so please make your reservations early.  This is
> their peak season and rooms go fast, but this rate is exceptional.  $119 
> for
> single, double, triple or quad.
>
> Come on down to Georgia April 13-15, 2007 to the Georgia Society for
> Histotechnology meeting.
>
> Shirley Powell
> GSH Secretary
>
> PS:  Check out the facilities at Mountain Creek Inn 
> www.callawaygardens.com.
> Call 1-800 225-5292 for Reservations.  Please state you are attending the
> GSH/ Meeting when making reservations in order to get the discounted rate 
> of
> $119 for single, double, triple or quad.
>
> Again, the deadline for reservations is March 1st to take advantage of 
> this
> rate.
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Fri, 9 Feb 2007 09:49:59 -0800 (PST)
> From: wen eng <weneng2004 <@t> yahoo.com>
> Subject: [Histonet] table for microscope
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <681386.52941.qm <@t> web55308.mail.re4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello,
>
> We are looking for a table for my microscope. We are moving to the 5th 
> floor and my microscope is Nikon E800. I was told I need to concern about 
> the vibration in tall building. So I want to ask the experts here what 
> kind of table is good enough for this.
>
> Thanks in advance.
> Wen
>
>
>
> ____________________________________________________________________________________
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> ------------------------------
>
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> End of Histonet Digest, Vol 39, Issue 15
> **************************************** 