[Histonet] Fwd: Osteoblast Staining

[patsy ruegg]

Another way to preserve alk phos enzyme in osteoblasts is to do frozen
sections on unfixed bone with the tape transfer system of course.  This
method yields so much alk phos it is over whelming.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of patsy ruegg
Sent: Thursday, February 08, 2007 4:05 PM
To: 'John Baker'; Histonet <@t> pathology.swmed.edu
Cc: ewaldorf <@t> engin.umich.edu
Subject: RE: [Histonet] Fwd: Osteoblast Staining

We had to process bone with cold methyl alcohol fixation into glycol
methacrylate (GMA) without decal to get the enzyme alk phos method to work.
It is also very sensitive to heat so you really have to avoid heat during
polymerization and other times.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Baker
Sent: Thursday, February 08, 2007 1:05 PM
To: Histonet <@t> pathology.swmed.edu
Cc: ewaldorf <@t> engin.umich.edu
Subject: [Histonet] Fwd: Osteoblast Staining

One of our graduate students has a question that I hoped someone in 
histonetland might be able to answer from their experience.
Thank you in advance,   John
>
> Subject: Osteoblast Staining
>
> I have been trying to do Alk.Phos. staining of paraffin embedded, EDTA 
> decalcified rat tibiae, using the Sigma kit #86 without success. I was 
> wondering if anyone has had any success with this? Also, I was 
> wondering if anyone had tried to do osteoblast staining with X-Gal, or 
> any other stain? Thank you for your assistance!   Erik Waldorff
>
>
>
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635
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