[Histonet] Re:Osteoblast staining
Rittman, Barry R
Barry.R.Rittman <@t> uth.tmc.edu
Thu Feb 8 16:07:21 CST 2007
I believe that with most types of fixation and processing that alkaline
phosphatase is destroyed.
However, if memory serves me correctly, a method was used in which
tissue was fixed in acetone and straight into wax.
Short time in wax.
Believe that it was stated that 70% of the alkaline phosphatase was
I did carry out this method on kidney and it was successful.
However, tissue is difficult to cut when fixed and processed in this
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
AGrobe2555 <@t> aol.com
Sent: Thursday, February 08, 2007 2:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re:Osteoblast staining
I don't have a good answer, but instead a question. Does Alkaline
phosphatase survive de-cal and paraffin processing? It seems that
activity would be killed by this processing.....
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation
Tissue Engineering Lab, Saint Patrick Hospital
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