[Histonet] Re:Osteoblast staining

[Rittman, Barry R]

I believe that with most types of fixation and processing that alkaline
phosphatase is destroyed.
However, if memory serves me correctly, a method was used in which
tissue was fixed in acetone and straight into wax.
Short time in wax.
Believe that it was stated that 70% of the alkaline phosphatase was
preserved.
I did carry out this method on kidney and it was successful.
However, tissue is difficult to cut when fixed and processed in this
manner.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
AGrobe2555 <@t> aol.com
Sent: Thursday, February 08, 2007 2:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re:Osteoblast staining


John,
I don't have a good answer, but instead a question.  Does Alkaline  
phosphatase survive de-cal and paraffin processing?  It seems that
all/most  enzymatic 
activity would be killed by this processing.....
Albert  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital


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