[Histonet] Fwd: Osteoblast Staining

[John Baker]

One of our graduate students has a question that I hoped someone in 
histonetland might be able to answer from their experience.
Thank you in advance,   John
>
> Subject: Osteoblast Staining
>
> I have been trying to do Alk.Phos. staining of paraffin embedded, EDTA 
> decalcified rat tibiae, using the Sigma kit #86 without success. I was 
> wondering if anyone has had any success with this? Also, I was 
> wondering if anyone had tried to do osteoblast staining with X-Gal, or 
> any other stain? Thank you for your assistance!   Erik Waldorff
>
>
>
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635