Fw: [Histonet] Ovary fixation

[Ian Montgomery]

Heidi,
            Ovaries, I normally fix using Bouin.
Ian.


Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Thomson Building,
University of Glasgow,
Tel:01413398855
Extn: 8511.
----- Original Message ----- 
From: "Heidi Miers" <Heidi.Miers <@t> NAU.EDU>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, February 06, 2007 6:41 PM
Subject: [Histonet] Ovary fixation


> I section dog and cat ovaries on a regular basis and am unhappy with the
> quality of the follicles. The primordial follicles are found near the
surface
> and when I look at them under the microscope they all look like they have
> vacuoles in the oocytes. Some ovaries come out looking OK. I am wondering
if
> anyone has suggestions. Could it be fixation? We use 10% formalin for at
least
> 3 days. How about processing? Could it be that the xylene substitute I use
> isn't clearing to allow proper paraffin infiltration?
> Also, for those who use a regressive H&E-what type of eosin and for how
long
> to you let it stain?
> Help!
> Thanks, Heidi
>
> Research Specialist, Sr.
> Imaging and Histology Core Facility
> Northern Arizona University
> Heidi.Miers <@t> nau.edu
> 928-523-9422
>
>
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