[Histonet] Staining frozen sections without fixation

[Martin S.]

Hi All,
 
I'm trying to get an aPDCA-1 antibody to work on frozen mouse spleen
sections. The antibody is normally used for FACS however the company
says that it works for immunhistochemistry. They recommend staining
before fixation. Usually I cut frozen sections, dry overnight (room
temp) and fix in acetone (10min at room temp) before putting the
sections in PBS and continuing with staining procedure.
 
If I stain before fixation;
 
Will the sections survive?
When should I fix - after primary, secondary etc?
What should I fix with?
 
Any help greatly appreciated.
 
Sonya