[Histonet] EBER

[patsy ruegg]

Rich,
This kind of use of automation after you have manually gone thru the steps
before hand, and I know that you look at the slides and have certain
expectations of performance, is the kind of automation that can help us move
forward.  When I express concerns about automation I am not talking about
this, I am talking about instances were there is no practical training
before using automation, and the users do not know what to expect or even
look to see what they got. 
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Sunday, February 04, 2007 9:43 AM
To: histonet; Patti Loykasek
Subject: Re: [Histonet] EBER

Hi Patti:

I have been very impressed with Vision BioSystems' EBER detection
technology.  We have been evaluating their EBER technology on their Bond Max
instrument and the results have been spectacular.  There are only two
reagents that you need in addition to their IHC detection kit; the
FITC-labeled EBER probe and an anti-FITC antibody.  Everything except
coverslipping is performed on the instrument.  I can't believe how easy it
is  to do.  I remember years ago when I had to "baby sit" the slides for the
better part of two days.  Now, I can get a result in approximately 4 hours
completely automated.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Patti Loykasek <ploykasek <@t> phenopath.com> 02/01/07 2:20 PM >>>
I have an inquiry for anyone doing EBV in-situ hybridization. What type of
probe are you using? How is your probe labeled? Are you using a kit & if so,
what kit? We are looking to optimize our sensitivity. Currently we are using
a digoxigen labeled EBV probe that we have made for us, detection is via an
anti-dig link and then Envision+-HRP, followed by DAB.
Thanks for the info.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA



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