[Histonet] EBER

[Richard Cartun]

Hi Patti:

I have been very impressed with Vision BioSystems' EBER detection technology.  We have been evaluating their EBER technology on their Bond Max instrument and the results have been spectacular.  There are only two reagents that you need in addition to their IHC detection kit; the FITC-labeled EBER probe and an anti-FITC antibody.  Everything except coverslipping is performed on the instrument.  I can't believe how easy it is  to do.  I remember years ago when I had to "baby sit" the slides for the better part of two days.  Now, I can get a result in approximately 4 hours completely automated.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Patti Loykasek <ploykasek <@t> phenopath.com> 02/01/07 2:20 PM >>>
I have an inquiry for anyone doing EBV in-situ hybridization. What type of
probe are you using? How is your probe labeled? Are you using a kit & if so,
what kit? We are looking to optimize our sensitivity. Currently we are using
a digoxigen labeled EBV probe that we have made for us, detection is via an
anti-dig link and then Envision+-HRP, followed by DAB.
Thanks for the info.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA



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