[Histonet] Histogel and microwave processing

Patsy Ruegg pruegg <@t> ihctech.net
Fri Feb 2 13:08:15 CST 2007


Actually I would worry about mw processing of histogel cell blocks as they
liquefy when heated.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Friday, February 02, 2007 11:58 AM
To: KirkyDog2 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Histogel and microwave processing

I don't know about mw processing of histogel cell blocks but I use a very
short cycle of 20 min per station starting with 70% etoh after the cell
block is well fixed which comes down to about 3.5 hrs. processing.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
KirkyDog2 <@t> aol.com
Sent: Friday, February 02, 2007 11:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Histogel and microwave processing

Hello everyone, I need help if anyone is willing.  We currently  process our

FNA cell blocks in "Histogel" using an overnight cycle, our  cytopathologist

would like his results faster.  Does anyone know if  histogel is compatible 
with microwave processing techniques, and if the cellular  morphology is 
compromised in any way with such a method?  Thank you in  advance for your
input.
T. Kirk
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