[Histonet] Re: IHC stainers Biogenex i6000

Mon Dec 31 02:53:26 CST 2007

Dear Netters,

I read a lot of information IHC autostainers here, but, what about Biogenex 
i6000? We have bought one and waiting for installation. It is a really cheap 
one.
Thanks in advance...

Dr. Necat Yilmaz

----- Original Message ----- 
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Sunday, December 30, 2007 8:21 PM
Subject: Histonet Digest, Vol 49, Issue 31

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> Today's Topics:
>
>   1. RE: bone holders (Gayle Callis)
>   2. Re:  Websites to demonstrate IHC methods (Gayle Callis)
>   3. RE: IHC stainers (pruegg <@t> ihctech.net)
>   4. IHC Stainers (Amos Brooks)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 29 Dec 2007 14:03:06 -0700
> From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] RE: bone holders
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001001c84a5e$35d59470$6501a8c0 <@t> DHXTS541>
> Content-Type: text/plain; charset="iso-8859-1"
>
> With a band saw, using pieces of 2 x 4 soft pine wood should hold the 
> bones.  You don't want the saw blade to hit a metal holder as the blade 
> may shatter and dulled, but worst of all is the potential for injury to 
> operator.  We used this method to saw through whole lengths of femurs of 
> calves\(huge!) and other animals when using a meat cutters band saw.   It 
> worked for human femoral heads too.   Simple grasp the bone between the 
> pieces of wood - a nice safe way to prevent losing a finger.  You can soak 
> the wood in bleach later, or simply discard for incineration.  The wood is 
> cheap and doesn't hurt the blade.   Try to have more teeth per inch in a 
> saw blade, you will get smoother, cleaner cuts,   We were able to saw with 
> water running on the blade, but that is not always possible.  However it 
> keeps the bone from being burned by high speed saws.
>
> Holding the bone this way allowed us to get precise 2 mm to 1 cm slabs for 
> doing large decalcified bone samples.
>
> Good luck
>
> Gayle M. Callis
> HT,HTL,MT(ASCP)
> Bozeman MT
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Sat, 29 Dec 2007 14:09:52 -0700
> From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] Re:  Websites to demonstrate IHC methods
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001701c84a5f$27548c20$6501a8c0 <@t> DHXTS541>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear K M,
>
> You can find IHC methods from just about any company selling antibodies 
> and their ancillary reagents.   As for animated IHC, you can find cartoons 
> for immunostaining on the Vector and DAKO websites, but not animated. 
> However they are step by step with explanation.   Go to www.IHCworld  and 
> look at all the methods found there.  If you are learning, this site is 
> superb for information.
>
> You can also access the DAKO handbook for immunostaining, and request a 
> copy of the last edition although I think it is available in pdf form on 
> the DAKO website.  They will send you a hard copy.
>
> Happy New Year
>
> Gayle M. Callis
> HT/HTL/MT(ASCP)
> Bozeman MT
>
> ------------------------------
>
> Message: 3
> Date: Sat, 29 Dec 2007 15:07:35 -0700
> From: <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] IHC stainers
> To: "'Larry Woody'" <slappycraw <@t> yahoo.com>, "'Sharon.Davis-Devine'"
> <Sharon.Davis-Devine <@t> carle.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <005101c84a67$386725b0$0202a8c0 <@t> ihctechq9h2qof>
> Content-Type: text/plain; charset="us-ascii"
>
> Sharon,
> If you are happy with the Dako, let them offer suggestions to up date your
> system, you will certainly have the most flexibility using a dako system 
> as
> it is completely open and much cheaper to use in my experience than being
> locked into detection systems for closed systems.  For more closed 
> systems,
> I have also heard really good things about Vision BioSystems (now
> LeicaBioSystems) machines.  Ask Dako and Leica what they can do for you,
> make them vie for your business, you will get the best deal if they think
> they have to compete.
> Patsy
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> email pruegg <@t> ihctech.net
> website www.ihctech.net
> IHC Resource Group www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Larry 
> Woody
> Sent: Friday, December 28, 2007 5:34 PM
> To: Sharon.Davis-Devine; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] IHC stainers
>
> Sharon:
>               I have used both the Ventana and the Lab Vision, (the one
> Dako distributes because the only one Dako makes is called Eridan and it
> doesn't work) ,and several others not worth mentioning, but if I had my
> choice I would probably have  several of each. If there are people with
> limited experience and knowledge in the area of IHC, then the Ventana is
> along those lines however if you are wanting to work up a novel antibody
> from scratch then you need an open platform where you can run an 
> experiment
> and the Lab Vision gives you that freedom.
>
> "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com> wrote:
>  I have recently been given the assignment of implementing a new and
> updated IHC system in our Histology department. We currently use a Dako
> Autostainer which we have used for several years and needs to be
> updated. I have limited in expertise in this are so am asking for all
> you Histonetters who have experience with this to give me some advice on
> which stainer to purchase. Let me know all of your thoughts both good
> and bad please. Thank you so much for your help.
>
>
>
> Sharon Davis-Devine, CT (ASCP)
>
> Cytology Supervisor
>
> Carle Clinic
>
> 602 West University
>
> Urbana, Illinois 61801
>
> Phone: 217-383-3572
>
> Email: sharon.davis-devine <@t> carle.com
>
>
>
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>
>
> Larry A. Woody
>  Seattle, Wa.
>
>
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>
> ------------------------------
>
> Message: 4
> Date: Sat, 29 Dec 2007 18:16:49 -0500
> From: "Amos Brooks" <amosbrooks <@t> gmail.com>
> Subject: [Histonet] IHC Stainers
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <582736990712291516o423bb64dge16d410c7933e786 <@t> mail.gmail.com>
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>
> Hi,
>     To delve into the mucky waters of this question is a slippery slope
> indeed. It honestly depends upon how much control you want to have (or to
> give up!) over the staining process and the chemicals you use. I must say
> that the Ventana is a FANTASTICALLY engineered bit of equipment. I would 
> use
> a Ventana in a second if it had the versatility I want in an autostainer.
> The ability to heat the slides individually and to stir the reagents is
> spectacular! Sadly unless you want to cough up a fortune for the high end
> machine, you are left having to use the secondary antibodies and 
> ancillaries
> they provide. I believe, (and I'm sure I'll be corrected if I'm wrong) the
> BondX is the same in this respect.
>     Hearing this, one may think that I would reccommend the DAKO and if 
> you
> read the archives you'd be right. Lately I have been less sure of that! 
> They
> have been working on the Eridan for a long time and I'm beginning to think
> it is a lost cause! (Think Vista from Microsoft) The development is long
> postponed and some say the company is going in the direction of the above
> equipment and attempting to lock users into a secondary detection system.
> The Original DAKO is still great and I still reccommend it, but I also
> reccommend the Thermo(FisherShandonRichard-AllenLabVision...) It is as, if
> not more versatile than the DAKO since it is based upon the same design.
>     My opinion is the open systems are better, but if closed works for you
> then my reasons for reccommending them are moot. As with all decisions in 
> a
> laboratory, it depends entirely on the needs of the laboratory.
>
> Good luck with this decision,
> Amos Brooks
>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 49, Issue 31
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