[Histonet] IHC for VIAS

[Gudrun Lang]

If you have the possibility minimize the block-size just for the tumor.
Score with a needle around the interesting areal and eliminate the fat. Be
sure, that no water is under the section, dry the slides upright.
We usually put the slides directly from cutting in the XT and have hardly
problems.

Gudrun Lang
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Godfrey
Guerzon
Sent: Thursday, December 20, 2007 4:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] IHC for VIAS

We are having problem with tissue falling off the slides when we run the
breast panel on the Ventana XT for examination with the VIAS instrument.
 The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides and
place them in 45 degree oven overnight and they still fall of.

Any suggestions?

Thanks.
          

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