[Histonet] IHC for VIAS

[Annette Hall]

We had the same problem with our XT. We now put the slides at 60 degrees for 1-2 hours. It's not perfect but we retain most of the tissue.

Annette J Hall, MT
Histo Supervisor
United Clinical Labs
Dubuque, Ia

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti Loykasek
Sent: Thursday, December 20, 2007 4:12 PM
To: Godfrey Guerzon; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC for VIAS

I would recommend some time in a 60-65 degree oven even if you do still want
to leave them in a 37-45 degree oven overnight.  That's just off the top of
my head - busy day here.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> We are having problem with tissue falling off the slides when we run the
> breast panel on the Ventana XT for examination with the VIAS instrument.
> The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides and
> place them in 45 degree oven overnight and they still fall of.
>
> Any suggestions?
>
> Thanks.
>
>
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