[Histonet] tissue burnt/dried on processor

Joe Nocito jnocito <@t> satx.rr.com
Tue Dec 11 06:07:29 CST 2007

I had  a problem with burnt tissue when I demo'ed a microwave tissue 
processor a couple of years ago. I cut the slides and used a HIER method 
just like on immunos. Some of the tissue stained better and some diagnosis's 
were able to be made, but some were totally lost.
Hope this helps

----- Original Message ----- 
From: "curt tague" <opiecurt <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, December 10, 2007 9:59 AM
Subject: [Histonet] tissue burnt/dried on processor

> to summarize, we had some of the reagents errantly changed on the tissue 
> processor. some of the 100% alc. seem to have been changed with 95% which 
> we thing resulted in water in the tissue through the xylene and wax. 
> however, the tissue was really dry and brittle.... i would really love to 
> resolve this problem rather than tell the clients that their tissue is 
> destroyed beyond repair. i have a picture i can forward to you if anyone 
> would like to take a look. basically, the epi, on skins specifically, is 
> staining really light. almost like we didn't leave it in there long 
> enough? pathologists were not happy to say teh least.
>  please help, let me know if i can forward the pic.
>  curt
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