[Histonet] tissue burnt/dried on processor
Kemlo.Rogerson <@t> waht.swest.nhs.uk
Mon Dec 10 10:21:45 CST 2007
Odd that, if the tissue was not fully dehydrated then the xylene
wouldn't have fully penetrated nor would the paraffin penetrated; it
ought to be soggy not 'dry and burnt'.
You could try revitalising by soaking in oil of cedar wood then
reprocessesing but that's usually for 'overprocessed' rather than
'underprocessed' material. Interesting xylene, if that's what you use,
can usually accommodate some water especially if fresh and clean (the
xylene that is)and I have known tissue be successfully processed despite
not using 100% and I wonder if the brittleness has been caused by
something else; Too much heat?
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