[Histonet] Freezing methode

[Reinders, N.R. (Niels)]

 Hello histosearch,

 

I was wondering if anyone ever heard about a freezingmethode where a
whole mouse-brain is placed is a cooled (nitrogen, dry-ice or
methylbutane) brain matrix? Will it freeze to it not to be removed in
one piece anymore? Or will it still change its shape in a matrix? I need
an alternative because I want less-experienced people to be able to
freeze the brain without much damage (I lost some brain lately). All
idea's are welcome!

 

Kind regards, N.Reinders

 

 

 

N.Reinders, molecular analist, 

University of Utrecht, NL

Yalelaan 2 3584 CM (363), Utrecht

(0031) (0)30 2533818

N.R.Reinders <@t> uu.nl <mailto:N.R.Reinders <@t> uu.nl>