[Histonet] Processing schedule for mouse tissue.

[Kim Merriam]

I have a VIP, so I don't know about your specific processor; but I would decrease your times to about 30 minutes per station for the first 3 alchohols and to a total of about 90 minutes for the 100% alchohol, xylene and paraffin (for example, 30-30-30, or something like that).  I am sure someone with your processor can chime in with a more specific mouse schedule.

Good luck!
Kim
 
Kim Merriam, MA, HT(ASCP)
Cambridge, MA



----- Original Message ----
From: Colin Nixon <c.nixon <@t> beatson.gla.ac.uk>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, December 10, 2007 3:30:56 AM
Subject: [Histonet] Processing schedule for mouse tissue.

Hi,

I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor.

70% Alcohol 35 mins
90% Alcohol 40 mins
95% Alcohol 45 mins
100% Alcohol 60 mins
100% Alcohol 60 mins
100% Alcohol 60 mins
Xylene 45 mins
Xylene 60 mins
Xylene 60 mins
Wax 90 mins
Wax 90 mins
Wax 90 mins

many thanks,

Colin 




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