[Histonet] Processing schedule for mouse tissue.

[Colin Nixon]

Hi,
 
I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor.
 
70% Alcohol 35 mins
90% Alcohol 40 mins
95% Alcohol 45 mins
100% Alcohol 60 mins
100% Alcohol 60 mins
100% Alcohol 60 mins
Xylene 45 mins
Xylene 60 mins
Xylene 60 mins
Wax 90 mins
Wax 90 mins
Wax 90 mins
 
many thanks,
 
Colin