[Histonet] Golgi slide bubbles

Bob Nienhuis bob.nienhuis <@t> gmail.com
Thu Dec 6 19:26:23 CST 2007

Doing Golgi-Cox staining of mouse brains and am having a problem with
bubbles appearing under the coverslip after va few days. I'm
guessing it's due to the solvent evaporating from the thick mounting
medium. Sections were cut frozen using the Kolb and McClimans (1986)
cryostat technique. 100 micron sections, DPX  mounting medium.

Any suggestions?

Bob Nienhuis

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