[Histonet] buffered verses unbuffered EDTA for decalcification for tunel

[Rene J Buesa]

EDTA in itself is a buffered solution once you equilibrate its pH during preparation.
  René J.

Liz Chlipala <liz <@t> premierlab.com> wrote:
  I need some help in determining if I need to use buffered or unbuffered
EDTA to decal samples for tunel. I have searched the histonet and there
are some protocols for buffered EDTA, but I have also seen protocols
that the EDTA is unbuffered. Any help is appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com 

Ship to Address:

Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.