[Histonet] buffered verses unbuffered EDTA for decalcification for tunel

Liz Chlipala liz <@t> premierlab.com
Wed Dec 5 12:45:57 CST 2007

I need some help in determining if I need to use buffered or unbuffered
EDTA to decal samples for tunel.  I have searched the histonet and there
are some protocols for buffered EDTA, but I have also seen protocols
that the EDTA is unbuffered.  Any help is appreciated.
Thanks in advance
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com <http://www.premierlab.com/> 
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

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