[Histonet] fresh kidney bx

Mitchell, Jeannette M. Jeannette.Mitchell <@t> vtmednet.org
Tue Dec 4 10:18:27 CST 2007

We have gotten freezing artifact when the isopentane is not cold enough. We charge the isopentane with some dry ice and freezing artifact disappears.  We also wash solution cat# 0103, Zeus Scientific and not PBS.

Jeannette Mitchell

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of eileen dusek
Sent: Tuesday, December 04, 2007 11:04 AM
To: histonet
Subject: [Histonet] fresh kidney bx

Hi Everyone,
  I have been in Histology for a looong time and have met my match. I recently have new duties involving kidney bxs. We receive a formalin, EM, and Michels (Zuess) fixed specimens.
  The Formalin and EM bx are not an issue, the Michels is kicking by butt!
  I transfer the specimen from Michels to PBS, to rinse the fixative, for about 2-3 mins of agitation. After the PBS the specimen goes into the first OCT. This is swirled for another 2-3mins. I repeat this process again.
  My problem is I get specimens that have too much freezing artifact. The structures are "blown up" and not compact.
  I appreciate any suggestions to help the problem         Thanks

  Eileen Dusek

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