[Histonet] massons trichrome

[kamal prasad]

Differentiate in phosphomolybdic-phosphotungstic acid solution for 15 minutes or until collagen and cytoplasm is not red.  Transfer sections directly (without rinse) to aniline blue solution and stain for 5-10 minutes. Rinse briefly in distilled water and differentiate in 1% acetic acid solution for 2-5 minutes.    
  Also I would suggest you to use another control to confirm(Fixation and Processing). 
   
  Regards,

       
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