[Histonet] RE: Histonet Digest, Vol 45, Issue 41

[Margaryan, Naira]

I do appreciate all of you who did answer on my question. I get very
good suggestions.

Thank you,
Naira 

-----Original Message-----
------------------------------

Message: 6
Date: Mon, 27 Aug 2007 16:34:11 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] ISH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<EE5AF506236CEE4CAFD1A281F5DCFE90A36C2C <@t> CMHEXC02EVS.childrensmemorial.or
g>
	
Content-Type: text/plain;	charset="us-ascii"

Hi histonetters,


I got a protocol for ISH for cells and there is a step, which looks very
strange for me: After cytospin cells on glass slides let them air dry at
RT for 60min. Is it not too long for the 60 min drying? Will cells
remain on the glass in this case or there is a risk pop off cells from
the glass?


Thanks in advance,
Naira

Naira V. Margaryan, D.V.M., Ph.D.
Children's Memorial Research Center
2300 Children's Plaza, Box 222
Chicago, IL 60614-3394
Tel: 773-755-6570/ext-8
Fax: 773-755-6594
nmargaryan <@t> childrensmemorial.org
<mailto:nmargaryan <@t> childrensmemorial.org> 

For Express Mail: 
CMRC, Room C.473
2430 N. Halsted Street
Chicago, IL  60614-4314