FW: [Histonet] buffer solutions

Ian Montgomery ian.montgomery <@t> bio.gla.ac.uk
Mon Aug 27 08:26:55 CDT 2007

	Dissolve the salts completely then make up to 1 litre for a Molar
solution. Dissolving the salts in 1 litre will give you a Molal solution.

Dr. Ian Montgomery,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
G12 8QQ.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise
Sent: 27 August 2007 10:19
To: Histonet <@t> lists.utsouthwestern.edu; kevin
Subject: [Histonet] buffer solutions

Hi all,

another laboratory controversy reigns!
When making up a buffer solution from salts what is the best practice: To
measure out the dry salt, place in beaker and then add the required volume
of water (ie up to 1liter) OR Measure out the 1liter and add the salts to
( I am asking in the context of making a 10x buffer soln, where the solute
weighs up to 150g)

Is there in fact any difference??

Best regards

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
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