[Histonet] alcohol fixation image (2nd try)

[patsy ruegg]

I think an important issue with alcohol fixation vs formalin is with IHC
differences, Gudrun did you compare IHC protocols on the formalin fixed vs
alcohol fixed samples?
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Garth Jerome
Sent: Tuesday, August 21, 2007 8:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] alcohol fixation image (2nd try)

Hello Gudrun

We recently ran an assay in conjunction with a local pathology laboratory,
to compare in detail, the difference cell morphology of formalin and
alchohol fixed / processed tissue. We are in the process of taking digital
images of the slides and will be uploading these to a website.

If you like, when done, I can try to arrange to send you a few.

Please advise if you would like me to do so.

Garth Jerome
Histologist
Apollo Scientific cc
Telephone : 27-11-466 7666
Fascimile : 27-11-466 7672
Email : garth <@t> apollosci.co.za

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: 18 August 2007 07:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] alcohol fixation image (2nd try)

Uups, should be "formalin and alcohol-fixed".

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Samstag, 18. August 2007 19:05
An: 'Geoff McAuliffe'
Cc: histonet <@t> lists.utsouthwestern.edu
Betreff: AW: [Histonet] alcohol fixation image (2nd try)

Geoff and Bryan,
Unfortunatly I have no access to these books. But I hoped someone could
describe in a few words the essential differences between formalin and
formalin-fixed tissue.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
Gesendet: Freitag, 17. August 2007 20:46
An: gu.lang <@t> gmx.at
Cc: histonet <@t> lists.utsouthwestern.edu
Betreff: Re: [Histonet] alcohol fixation image (2nd try)

I recall that an early edition (1960's or so) of one of the major 
histology texts (Bloom and Fawcett's, A Textbook of Histology I think) 
had drawing of how and intestinal epithelial cell would look after 
various fixatives. Also try some older editions (pre 1960's) of Maximow 
and Bloom, Bailey, Ham etc, all of the major histo texts that are too 
long and encyclopedic for today's medical student.

Geoff


Gudrun Lang wrote:
> Hi,
>
> I have to admit, that I need help with basic histotechnical knowledge.
> Please, can someone describe me the "alcohol fixation image" of tissue,
> especially chromatin? 
>
> And would poorly formalin-fixed tissue show less chromatin-structure or
more
> intense chromatin-structure, after the usual VIP-processing.
> (50-70-96-96-100-100 alc.) due to the ethanol-effect?
>
>  
>
> Thanks in advance
>
> Gudrun Lang
>
>
>
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> Histonet <@t> lists.utsouthwestern.edu
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>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************


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