AW: [Histonet] alcohol fixation image (2nd try)

[Geoff McAuliffe]

Dear Gudrun:

    Try a public library, university library or second-hand book store 
for old histology texts. Also see if you can find a copy of "Cytological 
Technique" or "Principles of Biological Microtechnique" both by John R. 
Baker. Classic works in the field.

Geoff

Gudrun Lang wrote:
> Geoff and Bryan,
> Unfortunatly I have no access to these books. But I hoped someone could
> describe in a few words the essential differences between formalin and
> formalin-fixed tissue.
>
> Gudrun Lang
>  
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
>
> -----Ursprüngliche Nachricht-----
> Von: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
> Gesendet: Freitag, 17. August 2007 20:46
> An: gu.lang <@t> gmx.at
> Cc: histonet <@t> lists.utsouthwestern.edu
> Betreff: Re: [Histonet] alcohol fixation image (2nd try)
>
> I recall that an early edition (1960's or so) of one of the major 
> histology texts (Bloom and Fawcett's, A Textbook of Histology I think) 
> had drawing of how and intestinal epithelial cell would look after 
> various fixatives. Also try some older editions (pre 1960's) of Maximow 
> and Bloom, Bailey, Ham etc, all of the major histo texts that are too 
> long and encyclopedic for today's medical student.
>
> Geoff
>
>
> Gudrun Lang wrote:
>   
>> Hi,
>>
>> I have to admit, that I need help with basic histotechnical knowledge.
>> Please, can someone describe me the "alcohol fixation image" of tissue,
>> especially chromatin? 
>>
>> And would poorly formalin-fixed tissue show less chromatin-structure or
>>     
> more
>   
>> intense chromatin-structure, after the usual VIP-processing.
>> (50-70-96-96-100-100 alc.) due to the ethanol-effect?
>>
>>  
>>
>> Thanks in advance
>>
>> Gudrun Lang
>>
>>
>>
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>>
>>   
>>     
>
>
>   


-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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