[Histonet] B&B Gram negative color change

[Rene J Buesa]

If you do not totally wash/remove/differentiate between steps, that could happen.
  René J.

"Woodward, Denise" <denise.woodward <@t> uconn.edu> wrote:
  
Hello all,

We recently noticed that our B&B positive controls change color over
time after staining. It happens in as little time as a week.
The Gram negative organisms change hue from a nice pink/red to a purple
color. The Gram positive organisms remain blue.
Anyone have any clues as to why/how this is happening?
Sorry to ask a difficult question on a Friday but this one has been
bothering me for a few days.
Thanks,
Denise Long Woodward
UConn
Dept. of Pathobiology and Veterinary Sciences
Storrs, CT

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