AW: [Histonet] Sensitivity and specificity

Gudrun Lang gu.lang <@t>
Fri Aug 17 03:02:25 CDT 2007

Sensitivity refers to the minimal ammount of antigen, that can be detected
by the whole detection-system (primary antibody qualitiy/quantity; secondary
antibody; activity of the used enzyme; etc.)
The sensitivity can be encreased by higher affine antibodies, more "steps",

The specifity of a test refers to the fact, that the test is only positiv,
if the tested substance is really present; the test must not be positiv with
any other substance. For example, if an antibody crossreacts with epitops on
various celltypes, the antibody and the test are not specific for a unique
cell-type. - but the antibody itself is specific to its epitop.

Hope this helps

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Laurie
Gesendet: Freitag, 17. August 2007 09:06
An: histonet <@t>
Betreff: [Histonet] Sensitivity and specificity



Can anyone help with a concise definition of "sensitivity" and "specificity"
as it relates to immunohistochemical reactions.


Thanks and regards,   Laurie.


Mr. Laurie Reilly

School of Veterinary & Biomedical Sciences

James Cook University


Queensland  4811



Phone  07 4781 4468

Fax  07 4779 1526


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