[Histonet] Cryo sections

[Pat Keller]

I am cutting adult rat brainstems, transcardially perfused with 4%
paraformaldehyde in PBS and immersed in same overnight at 4c then sunk in 30
sucrose in PBS cutting at 8 microns on cryostat  as they dry they rip
regards of the type of slide. What in the world am I doing wrong been
cutting for 25 yr. and this is a first for me. Box at -25c  using high
profile accu-edge blade, using both OCT and TBS no help still ripping
Took sample in paraffin good cut at 8 microns

Thanks
Pat Keller


Patricia Keller, 
Sr. Res. Tech.   Histology Core Manager
Washington University School of Medicine
Department of Otolaryngology
Campus box 8115
4566 Scott Ave.
St. Louis,   MO.   63110
KellerP <@t> ent.wustl.edu
http://www.otocore.org/

OFFICE    314-747-7166
FAX          314-747-7230

<br/>The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail.