[Histonet] RE: Histonet Digest, Vol 45, Issue 18
Bernice Frederick
b-frederick <@t> northwestern.edu
Tue Aug 14 14:59:11 CDT 2007
Labvision (now a part of Fisher)also sells it (galectin-3).
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Tuesday, August 14, 2007 2:15 PM
To: Danahy, Mary-Lee (R2); histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Histonet Digest, Vol 45, Issue 18
Hi Mary Lee. Is the antibody perhaps Galectin-3? Galectin-3 is useful in
distinguishing thyroid neoplasms. We are in the process of working up a
Galectin-3 antibody clone 9C4 from Vector Laboratories.
Hope this helps.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
> Help...again,
> Does anybody have any info. on calectin-3 ? What is it? Who uses it?
Where do
> you get it?
> Does anybody else use the Breast Receptor Block from Zymed ? If so how
do you
> find it works for you?
> Thanks for listening.
> Mary Lee Danahy R.T.,M.L.T.III
> Immunohistochemistry/Molecular Lab.
> Pathology Dept.
> Atlantic Health Sciences Corporation
> Saint John N.B.Canada
> tel.# 506-648-6604
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> histonet-request <@t> lists.utsouthwestern.edu
> Sent: Monday, August 13, 2007 2:02 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 45, Issue 18
>
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> Contents of Histonet digest..."
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>
> Today's Topics:
>
> 1. Starfrost slides IHC (Melinda Goodyear)
> 2. (no subject) (MARY T HODGES)
> 3. Re: mouse spinal cord atlas (Geoff McAuliffe)
> 4. Re: mouse spinal cord atlas (Emily Sours)
> 5. RELIA Special Job Alert for Managers, Supervisors and Lead
> Techs (Pam Barker)
> 6. Xylene substitutes (Kalleberg, Kristopher)
> 7. Re: mouse spinal cord atlas (Geoff McAuliffe)
> 8. Re: Xylene substitutes (Rene J Buesa)
> 9. Pre-filled containers of B-5 fixative (MICHELLE MCNEESE)
> 10. RE: Pre-filled containers of B-5 fixative (Weems, Joyce)
> 11. RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative
> (Douglas D Deltour)
> 12. Looking for book (Perry, Margaret)
> 13. Re: Looking for book (Jackie M O'Connor)
> 14. Re: Looking for book (Jackie M O'Connor)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 13 Aug 2007 17:27:50 +1000
> From: "Melinda Goodyear" <M.Goodyear <@t> latrobe.edu.au>
> Subject: [Histonet] Starfrost slides IHC
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <61B1A7B85B73F44B9DBCB9F16C8D1C8202A30AE8 <@t> EXCHANGE.ltu.edu.au>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all,
>
>
>
> I have been in contact with a third party from Australia that
distributes
> Starfrost slides made by Waldemar Knittel Glasbearbeitungs GmbH
(Knittel
> Glaser) as my tissue sections have been falling off during
> immunohistochemistry using a new batch of slides (Starfrost Adhesive
> hydrophilic slides) with the same catalogue number as previously
ordered. I
> received an explanation that all previous batches of slides sent may
have been
> mis-labelled and probably had an adhesive applied like silane coated
or
> poly-L-lysine. The only difference between the old batch of slides
and new
> batch is that the old ones have 2 stars printed at the top of the
slides and
> the boxes are labelled as "adhesive-Objekttrager" rather than
"Objekttrager".
> I am wondering whether anybody else may be have been having problems
with
> these slides and whether changing to silane coated may help?
>
>
>
> Thanks in advance for your help,
>
>
>
> Melinda
>
> Visual Neuroscience Lab
>
> School of Psychological Science
>
> Faculty of Science, Technology and Engineering
>
> La Trobe University
>
> Victoria, 3086
>
> PH: +61 3 9479 2470
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 13 Aug 2007 07:40:01 -0400
> From: "MARY T HODGES" <hodges420 <@t> msn.com>
> Subject: [Histonet] (no subject)
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY103-F3823228DC42DD507655EDFE1DC0 <@t> phx.gbl>
> Content-Type: text/plain; format="flowed"
>
>
> Has anyone had any trouble confirming their regristry from ASCP?
> I have completely dropped out of the system and don't know what or
who
> to contact.
> Tere Hodges
> Tucson, Az
> _________________________________________________________________
>
> [1]Booking a flight? Know when to buy with airfare predictions on
MSN
> Travel.
>
> References
>
> 1. http://g.msn.com/8HMAENUS/2755??PS=47575
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 13 Aug 2007 09:28:22 -0400
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] mouse spinal cord atlas
> To: Emily Sours <talulahgosh <@t> gmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <46C05C76.9020408 <@t> umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> Greetings all:
>
> The link Emily supplied does not work and I could not find "The
House Mouse"
> at amazon.com. "The Biology of the Laboratory Mouse", by Earl Green
(former
> director of the Jackson Lab) might have the information the original
poster
> was seeking.
>
> Geoff
>
> Emily Sours wrote:
>> The best atlas I have heard of dealing with the mouse spinal cord is
>> called "The House Mouse." It is no longer being published but the
>> amazon link is here tinyuRL.com/2rnvul You may want to see if it's
>> been updated with a more recent name. If not, try to find a used
copy
>> with bookfinder.com I haven't personally used this, but my boss
swears
>> by it, and she's an old school anatomist of mice and chicks.
>>
>> Emily
>>
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 13 Aug 2007 09:42:13 -0400
> From: "Emily Sours" <talulahgosh <@t> gmail.com>
> Subject: Re: [Histonet] mouse spinal cord atlas
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <b39794b0708130642u7be3337l5b34dc586c7d4b5c <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Here's a long link for The House Mouse at amazon
>
http://www.amazon.com/House-Mouse-Atlas-Embryonic-Development/dp/3540059
407/re
> f=sr_1_2/105-5355714-4615608?ie=UTF8&s=books&qid=1186808006&sr=8-2
> here's another try for the short one:
> http://www.tinyurl.com/2rnvul
>
>
> Emily
> --
> these things happen, you know, you go for a walk in the park one day
and
> wheelchair ninjas and nazis and pots-and-pans robots show up to kill
you and
> dinosaurs show up to eat the remains.
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 13 Aug 2007 10:41:13 -0400
> From: "Pam Barker" <relia1 <@t> earthlink.net>
> Subject: [Histonet] RELIA Special Job Alert for Managers,
Supervisors
> and Lead Techs
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E1IKb6Y-000234-6g <@t> elasmtp-curtail.atl.sa.earthlink.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello Histonetters,
> I have several exciting opportunities for experienced Histology
> Managers, Supervisors and Lead Technicians in hospital and private lab
> environments in several locations nationwide. The compensation
packages
> are excellent, the work is challenging and the sky is the limit. The
> positions are of course full time and day shift.
>
>
>
> Here are my leadership positions:
>
> Histology Manager - Central CA, Near Santa Barbara
>
> Histology Supervisor - Gulf Coast, Texas
>
> Histology Supervisor - Southeastern MA
>
> Histology Manager - Chicago, IL
>
>
> If you would like more information or know of someone else who might
be
> interested, please contact me at relia1 <@t> earthlink.net or 866-607-3542.
> I am available to discuss the opportunity at your convenience
including
> after hours. Thanks-Pam
>
>
> Thank You!
>
>
> Pam Barker
> President
> RELIA
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: relia1 <@t> earthlink.net
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 13 Aug 2007 11:15:53 -0400
> From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
> Subject: [Histonet] Xylene substitutes
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
<0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76 <@t> NTRSEVS30002.s3.ms.unilever.co
m>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Is anyone out there familiar with Xylene substitutes such as
> Histo-Clear? I am wondering if switching over the the substitutes is
a
> good idea. Any positive or negative feedback would be greatly
> appreciated when it comes to IHC results. Thanks in adavance.
>
> Kris
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 13 Aug 2007 11:14:55 -0400
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] mouse spinal cord atlas
> To: Kar Ling <karling <@t> usc.edu>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <46C0756F.6000507 <@t> umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> You might have a look at Progress in Brain Research 11:1-56, 1964 by
> Nieuwenhuys, a long paper on comparative anatomy of the spinal cord.
>
> Geoff
>
>
> Kar Ling wrote:
>> Hi,
>>
>> I have problems in defining ventral roots of lumbar spinal segments
in mice.
>> I try to look up some mouse anatomy book, but it only has bunch of
spinal
>> cord cross section. For gross anatomy of the spinal cord, I can only
find
>> rat ones. I wonder if there's such book with whole spinal cord
illustration.
>>
>> My first questions is: Do mice has L1-L6 and S1-S4 as in rat?
>>
>> My way to dissect spinal cord is cutting the dorsal bones piece by
pieces,
>> and then carefully remove the ventral bones starting at the sides so
that I
>> can preserve DRG attached to both ventral and dorsal roots. (that
took me an
>> hour to expose sacral and lumber segments).
>>
>> If I count from conus medullaris, how can I be sure my counting is
correct?
>> should I count main nerves or emerging branches? Is there any
hallmarks that
>> can differentiate L6 from S1?
>>
>> According to what I see in mouse spinal cord, the last thick ventral
spinal
>> nerve coming out from the spinal cord appears to be several branches
emerging
>> from the cord then merging into one, but then split into 2 again.
One of
>> those nerves attach another dorsal root and DRG, yet I fail to
preserve the
>> other DRG if any. As for the 2 ventral nerves below this one are
much
>> thinner and also has few emerging branches from the cord. So, I am
not sure
>> about what I see is L6 or it's a merge of L6 & S1??
>>
>> If I count the afforementioned nerve as L6, I can locate 3 pairs of
long
>> dorsal and ventral roots (L6-L3) and 2 pairs of relatively short
L2-L1 above.
>> Yet, L1 roots are notably longer than Thoracic root along rib cage
level. So,
>> Is L1 always longer than T12??
>>
>> I read some histonet achieve suggesting saline injection to push the
spinal
>> cord out, can the DRG be preserved?
>>
>> Thank you.
>>
>> Karen Ling
>> University of Southern California
>> Program in Neuroscience
>> 3641 Watt Way, HNB 209
>> Los Angeles, CA 90089-2520
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>
>
>
>
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