[Histonet] RE: Histonet Digest, Vol 45, Issue 18
Patti Loykasek
ploykasek <@t> phenopath.com
Tue Aug 14 14:15:25 CDT 2007
Hi Mary Lee. Is the antibody perhaps Galectin-3? Galectin-3 is useful in
distinguishing thyroid neoplasms. We are in the process of working up a
Galectin-3 antibody clone 9C4 from Vector Laboratories.
Hope this helps.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
> Help...again,
> Does anybody have any info. on calectin-3 ? What is it? Who uses it? Where do
> you get it?
> Does anybody else use the Breast Receptor Block from Zymed ? If so how do you
> find it works for you?
> Thanks for listening.
> Mary Lee Danahy R.T.,M.L.T.III
> Immunohistochemistry/Molecular Lab.
> Pathology Dept.
> Atlantic Health Sciences Corporation
> Saint John N.B.Canada
> tel.# 506-648-6604
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> histonet-request <@t> lists.utsouthwestern.edu
> Sent: Monday, August 13, 2007 2:02 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 45, Issue 18
>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific than "Re:
> Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Starfrost slides IHC (Melinda Goodyear)
> 2. (no subject) (MARY T HODGES)
> 3. Re: mouse spinal cord atlas (Geoff McAuliffe)
> 4. Re: mouse spinal cord atlas (Emily Sours)
> 5. RELIA Special Job Alert for Managers, Supervisors and Lead
> Techs (Pam Barker)
> 6. Xylene substitutes (Kalleberg, Kristopher)
> 7. Re: mouse spinal cord atlas (Geoff McAuliffe)
> 8. Re: Xylene substitutes (Rene J Buesa)
> 9. Pre-filled containers of B-5 fixative (MICHELLE MCNEESE)
> 10. RE: Pre-filled containers of B-5 fixative (Weems, Joyce)
> 11. RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative
> (Douglas D Deltour)
> 12. Looking for book (Perry, Margaret)
> 13. Re: Looking for book (Jackie M O'Connor)
> 14. Re: Looking for book (Jackie M O'Connor)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 13 Aug 2007 17:27:50 +1000
> From: "Melinda Goodyear" <M.Goodyear <@t> latrobe.edu.au>
> Subject: [Histonet] Starfrost slides IHC
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <61B1A7B85B73F44B9DBCB9F16C8D1C8202A30AE8 <@t> EXCHANGE.ltu.edu.au>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all,
>
>
>
> I have been in contact with a third party from Australia that distributes
> Starfrost slides made by Waldemar Knittel Glasbearbeitungs GmbH (Knittel
> Glaser) as my tissue sections have been falling off during
> immunohistochemistry using a new batch of slides (Starfrost Adhesive
> hydrophilic slides) with the same catalogue number as previously ordered. I
> received an explanation that all previous batches of slides sent may have been
> mis-labelled and probably had an adhesive applied like silane coated or
> poly-L-lysine. The only difference between the old batch of slides and new
> batch is that the old ones have 2 stars printed at the top of the slides and
> the boxes are labelled as "adhesive-Objekttrager" rather than "Objekttrager".
> I am wondering whether anybody else may be have been having problems with
> these slides and whether changing to silane coated may help?
>
>
>
> Thanks in advance for your help,
>
>
>
> Melinda
>
> Visual Neuroscience Lab
>
> School of Psychological Science
>
> Faculty of Science, Technology and Engineering
>
> La Trobe University
>
> Victoria, 3086
>
> PH: +61 3 9479 2470
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 13 Aug 2007 07:40:01 -0400
> From: "MARY T HODGES" <hodges420 <@t> msn.com>
> Subject: [Histonet] (no subject)
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY103-F3823228DC42DD507655EDFE1DC0 <@t> phx.gbl>
> Content-Type: text/plain; format="flowed"
>
>
> Has anyone had any trouble confirming their regristry from ASCP?
> I have completely dropped out of the system and don't know what or who
> to contact.
> Tere Hodges
> Tucson, Az
> _________________________________________________________________
>
> [1]Booking a flight? Know when to buy with airfare predictions on MSN
> Travel.
>
> References
>
> 1. http://g.msn.com/8HMAENUS/2755??PS=47575
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 13 Aug 2007 09:28:22 -0400
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] mouse spinal cord atlas
> To: Emily Sours <talulahgosh <@t> gmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <46C05C76.9020408 <@t> umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> Greetings all:
>
> The link Emily supplied does not work and I could not find "The House Mouse"
> at amazon.com. "The Biology of the Laboratory Mouse", by Earl Green (former
> director of the Jackson Lab) might have the information the original poster
> was seeking.
>
> Geoff
>
> Emily Sours wrote:
>> The best atlas I have heard of dealing with the mouse spinal cord is
>> called "The House Mouse." It is no longer being published but the
>> amazon link is here tinyuRL.com/2rnvul You may want to see if it's
>> been updated with a more recent name. If not, try to find a used copy
>> with bookfinder.com I haven't personally used this, but my boss swears
>> by it, and she's an old school anatomist of mice and chicks.
>>
>> Emily
>>
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 13 Aug 2007 09:42:13 -0400
> From: "Emily Sours" <talulahgosh <@t> gmail.com>
> Subject: Re: [Histonet] mouse spinal cord atlas
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <b39794b0708130642u7be3337l5b34dc586c7d4b5c <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Here's a long link for The House Mouse at amazon
> http://www.amazon.com/House-Mouse-Atlas-Embryonic-Development/dp/3540059407/re
> f=sr_1_2/105-5355714-4615608?ie=UTF8&s=books&qid=1186808006&sr=8-2
> here's another try for the short one:
> http://www.tinyurl.com/2rnvul
>
>
> Emily
> --
> these things happen, you know, you go for a walk in the park one day and
> wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and
> dinosaurs show up to eat the remains.
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 13 Aug 2007 10:41:13 -0400
> From: "Pam Barker" <relia1 <@t> earthlink.net>
> Subject: [Histonet] RELIA Special Job Alert for Managers, Supervisors
> and Lead Techs
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E1IKb6Y-000234-6g <@t> elasmtp-curtail.atl.sa.earthlink.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello Histonetters,
> I have several exciting opportunities for experienced Histology
> Managers, Supervisors and Lead Technicians in hospital and private lab
> environments in several locations nationwide. The compensation packages
> are excellent, the work is challenging and the sky is the limit. The
> positions are of course full time and day shift.
>
>
>
> Here are my leadership positions:
>
> Histology Manager - Central CA, Near Santa Barbara
>
> Histology Supervisor - Gulf Coast, Texas
>
> Histology Supervisor - Southeastern MA
>
> Histology Manager - Chicago, IL
>
>
> If you would like more information or know of someone else who might be
> interested, please contact me at relia1 <@t> earthlink.net or 866-607-3542.
> I am available to discuss the opportunity at your convenience including
> after hours. Thanks-Pam
>
>
> Thank You!
>
>
> Pam Barker
> President
> RELIA
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: relia1 <@t> earthlink.net
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 13 Aug 2007 11:15:53 -0400
> From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
> Subject: [Histonet] Xylene substitutes
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76 <@t> NTRSEVS30002.s3.ms.unilever.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Is anyone out there familiar with Xylene substitutes such as
> Histo-Clear? I am wondering if switching over the the substitutes is a
> good idea. Any positive or negative feedback would be greatly
> appreciated when it comes to IHC results. Thanks in adavance.
>
> Kris
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 13 Aug 2007 11:14:55 -0400
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] mouse spinal cord atlas
> To: Kar Ling <karling <@t> usc.edu>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <46C0756F.6000507 <@t> umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> You might have a look at Progress in Brain Research 11:1-56, 1964 by
> Nieuwenhuys, a long paper on comparative anatomy of the spinal cord.
>
> Geoff
>
>
> Kar Ling wrote:
>> Hi,
>>
>> I have problems in defining ventral roots of lumbar spinal segments in mice.
>> I try to look up some mouse anatomy book, but it only has bunch of spinal
>> cord cross section. For gross anatomy of the spinal cord, I can only find
>> rat ones. I wonder if there's such book with whole spinal cord illustration.
>>
>> My first questions is: Do mice has L1-L6 and S1-S4 as in rat?
>>
>> My way to dissect spinal cord is cutting the dorsal bones piece by pieces,
>> and then carefully remove the ventral bones starting at the sides so that I
>> can preserve DRG attached to both ventral and dorsal roots. (that took me an
>> hour to expose sacral and lumber segments).
>>
>> If I count from conus medullaris, how can I be sure my counting is correct?
>> should I count main nerves or emerging branches? Is there any hallmarks that
>> can differentiate L6 from S1?
>>
>> According to what I see in mouse spinal cord, the last thick ventral spinal
>> nerve coming out from the spinal cord appears to be several branches emerging
>> from the cord then merging into one, but then split into 2 again. One of
>> those nerves attach another dorsal root and DRG, yet I fail to preserve the
>> other DRG if any. As for the 2 ventral nerves below this one are much
>> thinner and also has few emerging branches from the cord. So, I am not sure
>> about what I see is L6 or it's a merge of L6 & S1??
>>
>> If I count the afforementioned nerve as L6, I can locate 3 pairs of long
>> dorsal and ventral roots (L6-L3) and 2 pairs of relatively short L2-L1 above.
>> Yet, L1 roots are notably longer than Thoracic root along rib cage level. So,
>> Is L1 always longer than T12??
>>
>> I read some histonet achieve suggesting saline injection to push the spinal
>> cord out, can the DRG be preserved?
>>
>> Thank you.
>>
>> Karen Ling
>> University of Southern California
>> Program in Neuroscience
>> 3641 Watt Way, HNB 209
>> Los Angeles, CA 90089-2520
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>
>
>
>
-------------------------------------------------------------------------
This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any
unauthorized review, use, disclosure or distribution is prohibited. If
you are not the intended recipient, please contact the sender by e-mail
and destroy all copies of the original message, or you may call PhenoPath
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
More information about the Histonet
mailing list