[Histonet] RE: Histonet Digest, Vol 45, Issue 18
Danahy, Mary-Lee (R2)
danma <@t> reg2.health.nb.ca
Tue Aug 14 09:19:06 CDT 2007
Help...again,
Does anybody have any info. on calectin-3 ? What is it? Who uses it? Where do you get it?
Does anybody else use the Breast Receptor Block from Zymed ? If so how do you find it works for you?
Thanks for listening.
Mary Lee Danahy R.T.,M.L.T.III
Immunohistochemistry/Molecular Lab.
Pathology Dept.
Atlantic Health Sciences Corporation
Saint John N.B.Canada
tel.# 506-648-6604
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, August 13, 2007 2:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 45, Issue 18
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Today's Topics:
1. Starfrost slides IHC (Melinda Goodyear)
2. (no subject) (MARY T HODGES)
3. Re: mouse spinal cord atlas (Geoff McAuliffe)
4. Re: mouse spinal cord atlas (Emily Sours)
5. RELIA Special Job Alert for Managers, Supervisors and Lead
Techs (Pam Barker)
6. Xylene substitutes (Kalleberg, Kristopher)
7. Re: mouse spinal cord atlas (Geoff McAuliffe)
8. Re: Xylene substitutes (Rene J Buesa)
9. Pre-filled containers of B-5 fixative (MICHELLE MCNEESE)
10. RE: Pre-filled containers of B-5 fixative (Weems, Joyce)
11. RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative
(Douglas D Deltour)
12. Looking for book (Perry, Margaret)
13. Re: Looking for book (Jackie M O'Connor)
14. Re: Looking for book (Jackie M O'Connor)
----------------------------------------------------------------------
Message: 1
Date: Mon, 13 Aug 2007 17:27:50 +1000
From: "Melinda Goodyear" <M.Goodyear <@t> latrobe.edu.au>
Subject: [Histonet] Starfrost slides IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<61B1A7B85B73F44B9DBCB9F16C8D1C8202A30AE8 <@t> EXCHANGE.ltu.edu.au>
Content-Type: text/plain; charset="us-ascii"
Hi all,
I have been in contact with a third party from Australia that distributes Starfrost slides made by Waldemar Knittel Glasbearbeitungs GmbH (Knittel Glaser) as my tissue sections have been falling off during immunohistochemistry using a new batch of slides (Starfrost Adhesive hydrophilic slides) with the same catalogue number as previously ordered. I received an explanation that all previous batches of slides sent may have been mis-labelled and probably had an adhesive applied like silane coated or poly-L-lysine. The only difference between the old batch of slides and new batch is that the old ones have 2 stars printed at the top of the slides and the boxes are labelled as "adhesive-Objekttrager" rather than "Objekttrager". I am wondering whether anybody else may be have been having problems with these slides and whether changing to silane coated may help?
Thanks in advance for your help,
Melinda
Visual Neuroscience Lab
School of Psychological Science
Faculty of Science, Technology and Engineering
La Trobe University
Victoria, 3086
PH: +61 3 9479 2470
------------------------------
Message: 2
Date: Mon, 13 Aug 2007 07:40:01 -0400
From: "MARY T HODGES" <hodges420 <@t> msn.com>
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY103-F3823228DC42DD507655EDFE1DC0 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"
Has anyone had any trouble confirming their regristry from ASCP?
I have completely dropped out of the system and don't know what or who
to contact.
Tere Hodges
Tucson, Az
_________________________________________________________________
[1]Booking a flight? Know when to buy with airfare predictions on MSN
Travel.
References
1. http://g.msn.com/8HMAENUS/2755??PS=47575
------------------------------
Message: 3
Date: Mon, 13 Aug 2007 09:28:22 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] mouse spinal cord atlas
To: Emily Sours <talulahgosh <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <46C05C76.9020408 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Greetings all:
The link Emily supplied does not work and I could not find "The House Mouse" at amazon.com. "The Biology of the Laboratory Mouse", by Earl Green (former director of the Jackson Lab) might have the information the original poster was seeking.
Geoff
Emily Sours wrote:
> The best atlas I have heard of dealing with the mouse spinal cord is
> called "The House Mouse." It is no longer being published but the
> amazon link is here tinyuRL.com/2rnvul You may want to see if it's
> been updated with a more recent name. If not, try to find a used copy
> with bookfinder.com I haven't personally used this, but my boss swears
> by it, and she's an old school anatomist of mice and chicks.
>
> Emily
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 4
Date: Mon, 13 Aug 2007 09:42:13 -0400
From: "Emily Sours" <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] mouse spinal cord atlas
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b39794b0708130642u7be3337l5b34dc586c7d4b5c <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Here's a long link for The House Mouse at amazon
http://www.amazon.com/House-Mouse-Atlas-Embryonic-Development/dp/3540059407/ref=sr_1_2/105-5355714-4615608?ie=UTF8&s=books&qid=1186808006&sr=8-2
here's another try for the short one:
http://www.tinyurl.com/2rnvul
Emily
--
these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains.
------------------------------
Message: 5
Date: Mon, 13 Aug 2007 10:41:13 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Special Job Alert for Managers, Supervisors
and Lead Techs
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1IKb6Y-000234-6g <@t> elasmtp-curtail.atl.sa.earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"
Hello Histonetters,
I have several exciting opportunities for experienced Histology
Managers, Supervisors and Lead Technicians in hospital and private lab
environments in several locations nationwide. The compensation packages
are excellent, the work is challenging and the sky is the limit. The
positions are of course full time and day shift.
Here are my leadership positions:
Histology Manager - Central CA, Near Santa Barbara
Histology Supervisor - Gulf Coast, Texas
Histology Supervisor - Southeastern MA
Histology Manager - Chicago, IL
If you would like more information or know of someone else who might be
interested, please contact me at relia1 <@t> earthlink.net or 866-607-3542.
I am available to discuss the opportunity at your convenience including
after hours. Thanks-Pam
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
------------------------------
Message: 6
Date: Mon, 13 Aug 2007 11:15:53 -0400
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] Xylene substitutes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76 <@t> NTRSEVS30002.s3.ms.unilever.com>
Content-Type: text/plain; charset="us-ascii"
Is anyone out there familiar with Xylene substitutes such as
Histo-Clear? I am wondering if switching over the the substitutes is a
good idea. Any positive or negative feedback would be greatly
appreciated when it comes to IHC results. Thanks in adavance.
Kris
------------------------------
Message: 7
Date: Mon, 13 Aug 2007 11:14:55 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] mouse spinal cord atlas
To: Kar Ling <karling <@t> usc.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <46C0756F.6000507 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
You might have a look at Progress in Brain Research 11:1-56, 1964 by
Nieuwenhuys, a long paper on comparative anatomy of the spinal cord.
Geoff
Kar Ling wrote:
> Hi,
>
> I have problems in defining ventral roots of lumbar spinal segments in mice. I try to look up some mouse anatomy book, but it only has bunch of spinal cord cross section. For gross anatomy of the spinal cord, I can only find rat ones. I wonder if there's such book with whole spinal cord illustration.
>
> My first questions is: Do mice has L1-L6 and S1-S4 as in rat?
>
> My way to dissect spinal cord is cutting the dorsal bones piece by pieces, and then carefully remove the ventral bones starting at the sides so that I can preserve DRG attached to both ventral and dorsal roots. (that took me an hour to expose sacral and lumber segments).
>
> If I count from conus medullaris, how can I be sure my counting is correct? should I count main nerves or emerging branches? Is there any hallmarks that can differentiate L6 from S1?
>
> According to what I see in mouse spinal cord, the last thick ventral spinal nerve coming out from the spinal cord appears to be several branches emerging from the cord then merging into one, but then split into 2 again. One of those nerves attach another dorsal root and DRG, yet I fail to preserve the other DRG if any. As for the 2 ventral nerves below this one are much thinner and also has few emerging branches from the cord. So, I am not sure about what I see is L6 or it's a merge of L6 & S1??
>
> If I count the afforementioned nerve as L6, I can locate 3 pairs of long dorsal and ventral roots (L6-L3) and 2 pairs of relatively short L2-L1 above. Yet, L1 roots are notably longer than Thoracic root along rib cage level. So, Is L1 always longer than T12??
>
> I read some histonet achieve suggesting saline injection to push the spinal cord out, can the DRG be preserved?
>
> Thank you.
>
> Karen Ling
> University of Southern California
> Program in Neuroscience
> 3641 Watt Way, HNB 209
> Los Angeles, CA 90089-2520
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 8
Date: Mon, 13 Aug 2007 08:18:22 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Xylene substitutes
To: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <491845.24283.qm <@t> web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Kristopher:
This very same subject has been subject of discussion for years in Histonet. You are better off by consulting Histonet Archieves than waiting for direct answers.
René J.
"Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com> wrote:
Is anyone out there familiar with Xylene substitutes such as
Histo-Clear? I am wondering if switching over the the substitutes is a
good idea. Any positive or negative feedback would be greatly
appreciated when it comes to IHC results. Thanks in adavance.
Kris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool.
------------------------------
Message: 9
Date: Mon, 13 Aug 2007 10:35:40 -0500
From: "MICHELLE MCNEESE" <MICHELLE.MCNEESE <@t> childrens.com>
Subject: [Histonet] Pre-filled containers of B-5 fixative
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <46C033FC020000290000A652 <@t> CNET3.CHILDRENS.COM>
Content-Type: text/plain; charset=US-ASCII
We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions:
1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5?
2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others.
Thanks
Michelle L McNeese, HT (ASCP)
Lead Tech, Histology
Department of Pathology
Childrens Medical Center
(214) 456-6146
Email: michelle.mcneese <@t> childrens.com
------------------------------
Message: 10
Date: Mon, 13 Aug 2007 11:39:50 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Pre-filled containers of B-5 fixative
To: "MICHELLE MCNEESE" <MICHELLE.MCNEESE <@t> childrens.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA11B <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="utf-8"
We use B-Plus fixative from BBC Biochemical.
There will never be a fixative as good as mercury, but we must move forward to a better environment or all become mad hatters. Our pathologists took it kicking and screaming, but they have adjusted now. The hardest thing is to pull a previous bone marrow case to compare and the difference is so drastic. But it is a matter of retraining and accepting the change.
Good luck!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of MICHELLE
MCNEESE
Sent: Monday, August 13, 2007 11:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pre-filled containers of B-5 fixative
We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions:
1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5?
2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others.
Thanks
Michelle L McNeese, HT (ASCP)
Lead Tech, Histology
Department of Pathology
Childrens Medical Center
(214) 456-6146
Email: michelle.mcneese <@t> childrens.com
_______________________________________________
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Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
------------------------------
Message: 11
Date: Mon, 13 Aug 2007 11:48:18 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative
To: "'MICHELLE MCNEESE'" <MICHELLE.MCNEESE <@t> childrens.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1187024400.718.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Michelle,
It is a simple switch. We use B-Plus from BBC biochemical. You can get the
20 containers. http://www.bbcus.com/products.html?pc=3&pid=58
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of MICHELLE
MCNEESE
Sent: Monday, August 13, 2007 10:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative
We are in the process of eliminating B-5 fixative from our facility;
however, it is not a quick change or easy change for us and our supplier of
the small pre-filled containers (Poly Scientific) will no longer fill orders
after October. While we have eliminated the bulk of our use, we do still
have a few specific biopsies for which we are trying to find a suitable
alternative. With that said, I have two questions:
1. Is there a place where I can still get small (20 ml) pre-filled
containers of B-5?
2. What are some of the alternatives that people use for kidney, liver,
bone marrow, and the occassional lymph node? We have managed to move our
heart and GI biopsies, but still need the others.
Thanks
Michelle L McNeese, HT (ASCP)
Lead Tech, Histology
Department of Pathology
Childrens Medical Center
(214) 456-6146
Email: michelle.mcneese <@t> childrens.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Mon, 13 Aug 2007 11:07:42 -0500
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] Looking for book
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E92F2E88FFFFC74FBCF5A7415AC5E29DD5DAFE <@t> ATLANTIS-EVS.jacks.local>
Content-Type: text/plain; charset="us-ascii"
I am looking for the book Antigen Retrieval Techniques:
Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang
Gu, Clive R. Taylor.
This book is not available in bookstores I've searched on the Web and
I,ve tried Google and it is not available there either. Would anyone
have a copy that I can purchase? I am studying for my QIHC
certification.
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
------------------------------
Message: 13
Date: Mon, 13 Aug 2007 11:18:02 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Looking for book
To: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFA3174F26.45203297-ON86257336.005979F4-86257336.005995D2 <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
You can get it through Amazon.com ISBN 1-881299-43-0. I just looked
and it's available.
JO'C
"Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2007 11:07 AM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Looking for book
I am looking for the book Antigen Retrieval Techniques:
Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang
Gu, Clive R. Taylor.
This book is not available in bookstores I've searched on the Web and
I,ve tried Google and it is not available there either. Would anyone
have a copy that I can purchase? I am studying for my QIHC
certification.
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Mon, 13 Aug 2007 11:19:42 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Looking for book
To: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF47915D7E.41D467DD-ON86257336.0059A88A-86257336.0059BCD8 <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Nevermind. I was wrong. It's not available. Sorry. Stupid Monday
alert.
"Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2007 11:07 AM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Looking for book
I am looking for the book Antigen Retrieval Techniques:
Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang
Gu, Clive R. Taylor.
This book is not available in bookstores I've searched on the Web and
I,ve tried Google and it is not available there either. Would anyone
have a copy that I can purchase? I am studying for my QIHC
certification.
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 45, Issue 18
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