[Histonet] mouse skin processing for paraffin embedding

Monfils, Paul PMonfils <@t> Lifespan.org
Fri Aug 10 11:34:27 CDT 2007

I believe the times you listed are too short for processing skin adequately. I suspect your sections are separating on the water bath?  This is because the fat has not been completely removed, and the paraffin has not adequately infiltrated.  When I process mouse skin, I use 3 hours per station - 70% ethanol x1; 95% ethanol x1; 100% ethanol x4; xylene x3; paraffin x4.  I use a VIP processor, with vacuum on all stations. I have no problem sectioning skin processed this way.

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