[Histonet] RE: Slide Staining Variability

Margaryan, Naira NMargaryan <@t> childrensmemorial.org
Thu Aug 9 13:43:44 CDT 2007


Hi Annette and histonetters,


I am having same problem constantly on the automated IHC staining
systems from LabVision. 

I do cleaning of machine after 2-3 running independently on number of

I clean machine, but do my staining manually; I can't waist tissue and


I do call company; they are very nice people, came several times, check,
but problem still there.


It is so frustrating:-(:-(:-(






Message: 10

Date: Thu, 9 Aug 2007 08:41:50 -0500 

From: Annette Hall <Annette_hall <@t> pa-ucl.com>

Subject: [Histonet] Slide Staining Variability

To: Histonet <@t> lists.utsouthwestern.edu


      <9FC023A4AB52BB4D87DC6456081A822C012B6054 <@t> mercury.pa-ucl.com>

Content-Type: text/plain




For those of you who use automated IHC staining systems, has anyone had

issues with part of the slide staining and the rest of the slide not? We

have adopted the policy of placing the control on with the patient
tissue as

a more comprehensive check of the system. However, we occasionally find

control is acceptable but the patient fails to even counterstain. This

been an intermittent problem on the Ventana Benchmark for some time, but

frequency has been increasing to almost daily.


Any thoughts?



Annette J Hall

Histo/Cyto/Micro Supervisor

United Clinical Laboratories

Dubuque, IA 52001


-----Original Message-----

From: Harrison, Sandra C. [mailto:Sandra.Harrison3 <@t> va.gov] 

Sent: Monday, August 06, 2007 3:35 PM

To: Histonet <@t> lists.utsouthwestern.edu

Subject: [Histonet] Leica autostainer w/integrated coverslipper



Could anyone tell me if they're using the Leica AutoStainer XL (ST5010)

with the integrated coverslipper (CV5030)?

Also, can you tell me if you've experienced any trouble with it and how

long you've been using it?





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