AW: [Histonet] fixation-question

[Gudrun Lang]

I have found the statements in Luna's "Histopathologic methods and color
atlas of special stains and tissue artefacts" (1992) in the chapter
"fixation".

Formerly I have read your book, which impressed me very much. And after
learning much about the staining-theory and pH,  I thought Luna's statements
were wrong or at least not understandable for me.

What do you think?

 

Gudrun Lang

 

Biomed. Analytikerin

Histolabor

Akh Linz

Krankenhausstr. 9

4020 Linz

+43(0)732/7806-6754

  _____  

Von: John Kiernan [mailto:jkiernan <@t> uwo.ca] 
Gesendet: Dienstag, 07. August 2007 08:00
An: gu.lang <@t> gmx.at
Cc: histonet <@t> lists.utsouthwestern.edu
Betreff: Re: [Histonet] fixation-question

 

Have you read and compared the statements you  claim to have found "in the
literature"?

 

 

----- Original Message -----
From: Gudrun Lang <gu.lang <@t> gmx.at>
Date: Monday, August 6, 2007 14:19
Subject: [Histonet] fixation-question
To: histonet <@t> lists.utsouthwestern.edu

> Hi to all fixation-experts,
> 
> I've found in the literature some controversy statements about
> formaldehyd-fixation and want your help, what is the up-do-date-
> knowledge.
> -          
> Formaldehyd can/cannot be washed out of fixed tissue.
> 
> -          
> Formaldehyd fixates slower in more acid pH; uncharched
> aminogroups react better than protonated with formaldehyd. And 
> these groups
> should be uncharged in neutral pH.
> 
> -          Nuclear 
> chromatin is fixed only by acid fixatives.
> 
>  
> 
> What is your opinion on these statements?
> 
>  
> 
> Regards
> 
> Gudrun Lang
> 
>