[Histonet] fixation-question

[John Kiernan]

Have you read and compared the statements you  claim to have found "in the literature"?

 

----- Original Message -----
From: Gudrun Lang <gu.lang <@t> gmx.at>
Date: Monday, August 6, 2007 14:19
Subject: [Histonet] fixation-question
To: histonet <@t> lists.utsouthwestern.edu

> Hi to all fixation-experts,
> 
> I've found in the literature some controversy statements about
> formaldehyd-fixation and want your help, what is the up-do-date-
> knowledge.
> -          
> Formaldehyd can/cannot be washed out of fixed tissue.
> 
> -          
> Formaldehyd fixates slower in more acid pH; uncharched
> aminogroups react better than protonated with formaldehyd. And 
> these groups
> should be uncharged in neutral pH.
> 
> -          Nuclear 
> chromatin is fixed only by acid fixatives.
> 
>  
> 
> What is your opinion on these statements?
> 
>  
> 
> Regards
> 
> Gudrun Lang
> 
>